雷藤氯内酯醇对正常人及类风湿关节炎患者周围血单个核细胞及滑膜细胞产生免疫球蛋白的影响

EFFECTS OF TRIPCHLOROLIDE(T 4) OF TRIPTERYGIUM WILFORDII HOOK ON THE PRODUCTION OF IMMUNOGLOBULINS BY PERIPHERAL BLOOD MONONUCLEAR CELLS AND BY SYNOVIAL CELLS OF RHEUMATOID ARTHRITIS PATIENTS IN VITRO

研究了１０例正常人和６例类风湿关节炎（ＲＡ）患者外周血单个核细胞（ＰＢＭＣ）及３例ＲＡ患者消化的单个滑膜细胞（ＤＳＳＣ），经雷公藤的有效单体雷藤氯内酯醇（Ｔ４）处理２ｈ对其分泌免疫球蛋白（Ｉｇ）的影响。结果显示：Ｔ４在浓度为５～３５ｎｇ·ｍｌ－１对正常人ＰＢＭＣ分泌Ｉｇ有明显抑制作用，呈剂量依赖趋势。在２５ｎｇ·ｍｌ－１浓度下，Ｔ４对ＲＡ患者ＰＢＭＣ及ＤＳＳＣ分泌Ｉｇ均有明显抑制作用。

Tripchlorolide(T 4) is an active ingredient recently isolated from Tripterygium Wilfordii Hook. The in vitro effects of T 4 on the peripheral blood mononuclear cells(PBMC) of healthy persons( n =10), the digested single synovium cells(DSSC)( n =3) and PBMC( n =6) from RA patients on production of immunoglobulins(Ig) were studied using Elisa method. The results showed that T 4 at concentrations from 5 ng·ml -1 to 35 ng·ml -1 significantly reduced the production of Ig by PWM stimulated PBMC of healthy persons in a dose dependent manner. At concentration of 25 ng·ml -1 , T 4 also reduced the Ig secretion of RA PBMC and DSSC. It is hoped that T 4 would be an encouraging new drug of herbal nature for the treatment of RA.