不同工艺提取栀子黄色素的比较研究

Comparison of the Different Methods to Extract Yellow Pigment from Gardenia jasminoides Ellis

比较研究了热提取、超声波提取、微波提取3种不同工艺对栀子黄色素提取效果的影响。热浸提正交试验的最佳工艺条件:70%的乙醇(体积分数,下同)提取1.5 h,提取温度30℃,料液比1∶25(g∶mL,下同),产品的色价为66.45。超声波提取正交试验的最佳工艺条件:80%的乙醇提取20 min,料液比1∶20,产品的色价为87.77。微波提取单因素试验的最佳工艺条件:提取功率800 W,用50%乙醇提取30 min,提取温度50℃,料液比1∶5,色价为64.14。结果表明:热浸提、超声波提取、微波提取3种方法提取栀子黄色素的色价明显不同,超声波提取的效果最好。

The effects of extracting yellow pigment from Gardenia jasminoides Ellis by hot extraction, ultrasonic extraction and microwave-assisted extraction were compared. Orthogonal tests were used to optimize the conditions. Through orthogonal test of heat extraction, the optimum conditions were determined as follows：70 % EtOH as extracting solvent, 2 h, 30℃, 1 ： 25 solid-liquid ratio, the color value of the extracted pigment achieved 66.45. Through orthogonal test of ultrasonic extraction, the optimum conditions were determined as follows： 80 % EtOH as extracting solvent,20 min at 20℃, and solid-liquid ratio 1：20, the color value of the extracted pigment achieved 87.77. Through single factor test of microwave-assisted extraction, the optimum conditions were determined as follows： 800 W, 50 % EtOH as extracting solvent,30 min at 50℃, and solid-liquid ratio 1：5, the color value of the extracted pigment achieved 64.14. The results showed that the effects of extraction while by three different methods were different, ultrasonic extraction was the best way.