超声波辅助提取旱柳树叶中的总黄酮

Ultrasonic-assisted Extraction of Flavonoids from Leaves of Salix matsudana Koidz.

以旱柳落叶为原料,60%乙醇(质量分数,下同)为提取剂,芦丁为标准对照品,研究探索了柳叶中总黄酮的溶剂提取法和超声波辅助提取法的最佳提取条件,并对两种提取方法的提取效果进行了比较。试验表明,溶剂提取法的最佳提取条件为:0.3 g柳叶粉末以60%乙醇为溶剂,料液比1∶35(g∶mL,下同),65℃提取3次,每次75 min,总黄酮得率3.83%;超声波辅助提取的最佳条件为:0.3 g柳叶粉末以60%乙醇为溶剂,料液比1∶35,超声波功率400 W,超声波处理3次,每次30 min,总黄酮得率4.26%。超声波辅助提取比单纯的溶剂提取缩短了60%的提取时间,得率却提高了11.23%。

Fallen leaves of Salix matsudama Koidz. were used as raw material to extract flavonoids by using 60 % ethanol （ mass fraction） as solvent, using rutin as reference substance. Optimum conditions of solvent extraction and ultrasonic-assisted extraction were investigated and the extracting effects of two methods were compared. The results show that optimum conditions of solvent extraction are as follows ： fallen leaves powder 0.3 g, ethanol mass fraction 60 % , ratio of material to liquid 1：35 （g： mL） , extracting 3 times at 65℃, and 75 min for each time. Yield of flavonoids is 3.83 % under the conditions. Optimum conditions of ultrasonic-assisted extraction are： fallen leaves powder 0.3 g, ethanol mass fraction 60 % , the ratio of material to liquid 1：35 （g： mL） , the ultrasonic power 400 W, the ultrasonic wave treatment for twice and 30 min for each time, yield of flavonoids is 4.26 % under the conditions. The extracting time of ultrasonic-assisted extraction is 60 % shorter than solvent extraction, while yield of flavonoids is 11.23 % higher.