镉对雄性大鼠颌下腺超微结构的影响及丙酸睾酮的保护作用

The Effects of Cadmium on Ultrastructure of Submandibular Gland and the Protective Effects of Testosterone in Male Rats

目的探讨氯化镉对雄性大鼠颌下腺超微结构的影响及丙酸睾酮的保护作用。方法35只雄性Wistar大鼠分为3组:对照(C)组、镉(Cd)组和镉加丙酸睾酮(Cd+T)组。利用透射电镜作颌下腺组织颗粒曲管(GCT)细胞的超微结构观察。结果镉注射后24h,GCT细胞内出现不同程度的线粒体肿胀和(或)空泡化,胞质和线粒体内可见髓样结构,GCT周围毛细血管内皮细胞线粒体肿胀。7～15d后上述改变明显加重,部分GCT细胞见胞质广泛肿胀和轻度核溶解,30d后细胞形态仍未恢复正常。Cd+T组的超微结构损伤较相应Cd组轻。结论镉对GCT细胞的超微结构有早期直接损伤作用,后期雄激素减少加重此损伤,补充雄激素对上述损伤有一定的保护效果。

Objective To investigate the effects of cadmium and the protection of testosterone propionate on ultrastructure of submandibular gland of male rats. Methods Thirtyfive male adult Wistar rats were randomly divided into three groups： control, Cd and Cd ＋ T. The morphologic changes of GCT cells were observed by transmission electron microscopy. Results The mainly ultrastructural changes after Cd treatment included swelling or vacuolation of mitochondria, increase of myelin figures in GCT cells, and swelling of mitochondria in the endothelial cells of peritubular capillaries. The changes above were most prominent at 7 - 15d, and swelling of the cytoplasm and slight karyolysis were also found. The morphology of GCT cells didn＇ t recovery to normal yet after 30d. On the other hand, the ultrastructural lesions of GCT cells in Cd ＋ T group were gentle. Conchmions The present results indicate that the earlier ultrastructural damage of GCT was related to the direct action of cadmium, the decrease of androgen might aggravate the damage at later stage. To some extent, testosterone pretreatment could prevent toxicity of cadmium on submandibular gland.