异丙酚对脊髓缺血再灌注大鼠脊髓自由基和超氧化物歧化酶的影响

Effect of Propofol on Free Radical and SOD after Ischemia Reperfusion of Spinal Cord in Rat

目的研究异丙酚预处理对脊髓缺血再灌注早期脊髓组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性变化的影响,以探讨异丙酚在抗脊髓继发性损伤中的作用。方法54只成年雄性SD大鼠随机分为3组:假手术组(S)、再灌注组(IR)和异丙酚组(P),再灌注组和异丙酚组又各分1h、4h、8h、16h四个观察相点组(各组n=6)。除假手术组外,所有动物均采用腹主动脉肾下夹闭法造成脊髓缺血再灌注模型,观察各组脊髓组织内MDA含量和SOD活性。结果脊髓缺血再灌注后脊髓组织MDA立即上升,8h达最高峰,此后随之下降,但至16h仍高于正常对照组;而SOD活性显著下降,8h达最低,随后逐渐回升,至16h仍低于缺血前。与再灌注组比较,异丙酚组各相点MDA含量降低,SOD活性增强。结论异丙酚预处理对脊髓缺血再灌注损伤具有保护作用。

Objective To investigate the effects of propofol preconditioning on concentration of MDA and activities of superoxide dismutas （SOD） after spinal cord ischemia reperfusion associated with abdominal aortic crossclamping and explore the antioxidation of propofol. Methods 54 adult male Sprague - Dawley rats were randomly divided into sham group, ischemia reperfusion group and propofol group. The concentration of malonyldialdehyed （MDA） and activities of SOD in spinal cord were measured at pre - ishemia, repeifusion lh, repeifusion 4h, reperfusion 8h, reperfusion 16h. Results After referfusing, the concentration of MDA immediately increased till the peak at reperfusion 8h then decreased gradually, activities of SOD decreased and it reached the lowest at 8h then increased gradually. In propofol group, the concentrations of MDA were lower and the activities of SOD were higher at each phase point than these of ischemia reperfusion group. Conclusion This study suggested that propofol preconditioning had antioxidation in the secondary pathological damage of spinal cord ischemia repeifusion.