前列腺特异抗原基因启动子中二个与雄激素调节相关的序列

Two Androgen-related Regulatory Sequences in the Promoter Region of Prostate-Specific Antigen Gene

人前列腺特异抗原（ＰＳＡ）基因特异地在前列腺上皮细胞中表达。且受雄激素调节。其雄激素应答元件（ＡＲＥ）位于－１７０附近。为确定雄激素对该基因的诱导作用是否受ＡＲＥ上游序列的影响，把ＰＳＡ启动子区的不同长度的ＤＮＡ片段与无启动子的报告基因ＣＡＴ相连，然后与雄激素受体表达质粒一起共传染人前列腺肿瘤细胞ＰＣ－３。结果表明ＡＲＥ上游ＲＦ３６（－４０６～─３７１）和ＲＦ１５（─３４０～─３２６）两个序列可促进雄激素对ＰＳＡ基因的诱导作用。这些序列可与ＰＣ－３细胞中的某些调节蛋白结合。这些调节蛋白可能通过与雄激素受体的相互作用影响雄激素对ＰＳＡ基因的诱导作用。

Human prostate- specific antigen . PSA ) gene has been shown to be regulated by androgen , and its androgen reponse element (ARE) has been located at about position- 1 70 , To determine whether androgen induction of the gene was affected by upstream sequence of ARE . different DNA fragment of PSA were linked to a promoterless CAT reporter gene and cotransfected with an androgen receptor expression vector in human prostate cell line , PC-3. The results indicated that RF36 (─ 406─ ─ 371 ) and RF15 (─ 340─ ─ 326 ) sequences at the upstream of ARE could enhance androgen induction significantly. Some regulatory proteins fron PC-3 cells could bind at above sequences , which may interact with androgen receptor , promote androgen induction of PSA gene.