摘要
目的:建立恢复种植体周围骨缺损的自体骨碎末骨移植材料的实验模型。方法:实验于2005-08/2006-04在大连医科大学动物实验基地(辽宁省重点实验室)完成。①实验材料:健康杂交家犬5只,体质量15~20kg。Bio-Oss骨移植材料为引导骨/组织再生多孔骨无机材料,白色颗粒状,颗粒大小1.0~2.0mm。②实验方法:拔除家犬下颌第1,2,3前臼齿,3个月后行种植术。预备种植体窝,每只犬左右两侧各预备4个,共40个。在每个种植窝内,各植入种植体钛钉1枚,共40枚。用种植转孔时收集的自体骨碎末、Bio-Oss骨移植材料及两者1∶1混合骨碎末恢复种植体颊侧单壁人为骨缺损,以未植骨作空白对照。③实验评估:第9周时观察各组骨量的恢复情况、X线片观察牙槽骨高度、骨小梁致密度及骨整合情况,亚甲基蓝-碱性品红法观察组织学变化。结果:5只家犬钛钉均无脱落,均纳入结果分析。①一般情况:9周时,创口愈合均良好,钛钉稳定,总存留率为100%。骨缺损处已有不同程度恢复,与正常骨组织无明显差别。②9周时各组骨缺损量的测量结果:植入自体骨碎末、Bio-Oss骨移植材料、混合骨碎末及空白对照组的平均骨缺损量分别为1.8125、1.6975、1.5025、2.6375mm。植入混合骨碎末的平均骨缺损量最小,说明恢复最佳。③X线观察骨量的恢复情况:40颗钛钉外周均与骨组织紧密接触,愈合良好。不同组间未见明显骨质密度区别。④组织学观察骨量的恢复情况:低倍镜下见所有钛钉均被周围淡红色的致密骨组织紧密包绕,种植体与骨组织间无蓝色的软组织,产生了直接骨结合界面。结论:应用家犬建立自体骨碎末移植材料恢复种植体周围骨缺损的实验模型效果理想。
AIM: To repair bone defect with autogenous bone powder and implant material in experimental models. MEIHODS: The experiment was performed at the Animal Experimental Center (Liaoning Provincial Key Laboratory) of Dalian Medical University from August 2005 to April 2006. (1)Five healthy hybrid dogs with the body mass of 15-20 kg were selected. Bio-Oss bone implant material was porous inorganic material for guiding bone regeneration, showing white 1.0-2.0 mm granule. (2)The first, second and third premolars of dogs were uprooted, and then received implantation 3 months later. Four implant sockets were prepared at left and right sides respectively of each dog, totally 40 sockets. One Ti nail was implanted in each implant socket, totally 40 nails. Autogenous bone powder when drilling a hole, Bio-Oss implant materials and their mixture at 1:1 were implanted in artificial bone defect at bucca side, respectively and non-implanted bone as blank control. (3)At week 9, recovery of bone mass was recorded. Height of alveolar bone, density of bone trabecula and osseointegration were observed with X-ray film. Histological change was determined by methylene blue-basic fuchsin method. rlsults: No Ti nail fell off, so 5 dogs were involved in the result analysis. (1)At week 9, wound was well healed, and the Ti nail was stable with total reserved rate of 100%. Recovery to different extents appeared at bone defect region, and no significant difference was found compared with the normal bone. (2)At week 9, mean bone defect mass was 1.812 5, 1.697 5, 1.502 5 and 2.637 5 mm of bone implanted with autogenous bone powder, Bio-Oss implant materials, their mixture and blank control group, respectively. Mean bone defect mass was the least in bone implanted with mixed powder, which had optimal recovery. (3)The 40 Ti nails were closely connected with bone and the healing was well, No significant difference was found in bone density among different groups. (4)ALL Ti nails were closely surrounded by erythroic compact bone, no blue soft tissues were seen between implant and bone, and direct bone interface appeared under low power lens. CONCLUSION: It is effective to repair bone defect by autogenous bone powder and implant material in dog models.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第41期8221-8225,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research