后肢固定大鼠腓肠肌酶活性与银杏叶提取物EGB761的影响

Effects of ginkgo biloba extract 761 on ATPase activity of gastrocnemius in rats with fixed hindlimbs

目的:观察银杏叶提取物EGB761对后肢固定大鼠腓肠肌Na+-K+-ATP酶及Ca2+-ATP酶活性的影响,探讨其对失用性骨骼肌萎缩的防治作用。方法:实验于2005-04/2006-03在广州南方医院创伤骨科实验室(广东省重点实验室)完成。①实验分组:雄性SD大鼠24只,体质量200～260g,随机数字表法分为空白组、对照组、实验组,每组8只。②实验方法:制备大鼠骨骼肌失用性萎缩模型,将大鼠右后肢用可塑性石膏由足跖管型固定至膝上1cm处,膝关节固定于屈曲位100°,踝关节固定于跖屈60°,对照组给予蒸馏水3mL/d灌胃,实验组给予银杏叶提取物EGB761水溶液灌胃(灌胃剂量120mg/(kg·d),浓度14g/L)。③实验评估:4周后切取右后肢腓肠肌,去除腱膜、肌腱和神经,进行酶活性检测。结果:纳入大鼠24只,均进入结果分析。与空白组相比,对照组和实验组腓肠肌Na+-K+-ATP酶及Ca2+-ATP酶活性显著下降(P<0.05)。与对照组相比,实验组Na+-K+-ATP酶及Ca2+-ATP酶活性显著高于对照组(P<0.05)。结论:银杏叶提取物EGb761能够显著提高失用性萎缩骨骼肌Na+-K+-ATP酶及Ca2+-ATP酶活性,从而有效抑制骨骼肌失用性萎缩的发生。

AIM： To observe effects of extract of ginkgo biloba 761 （EGB761） on Na^＋-K^＋-ATPase and Ca^2＋-ATPase activities of gastrocnemius on fixed rat hindlimbs and study its preventing and treating effects on disused skeletal muscle atrophy. METHODS： The experiment was performed at the Laboratory of Department of Orthopaedics and Traumatology, （Key Laboratory of Guangdong Province） Nanfang Hospital from April 2005 to March 2006. （1）Totally 24 male SD rats with the body mass of 200-260 g were divided into blank group, control group and experimental group by the method of random digits table with 8 in each group. （2）Right hind limbs of rats were fixed with compliant plaster from metatarsus to 1 cm upper knees, knee joint was fixed in 100° and ankle joint in 60° extension position to establish disused skeletal muscle atrophy models. Rats in the control group were given with 3 mL per day distilled water by gastric perfusion, and rats in the experimental group were treated with EGB761 （120 mg/kg per day, 14 g/L） by gastric perfusion. （3）Gastrocnemius muscle in right hind limb was dissected fully 4 weeks later and aponeurotic membrane, tendon and nerve were removed for Na^＋-K^＋-ATPase and Ca^2＋-ATPase activities determination. RESULTS：A total of 24 rats were involved in the result analysis. Compared with blank group, Na^＋-K^＋-ATPase and Ca2＋-ATPase activities of gastrocnemius muscle were prominently lower in the control group and experimental group （P 〈 0.05）, and compared with the control group, Na^＋-K^＋-ATPase and Ca^2＋-ATPase activities of gastrocnemius muscle were significantly higher in the experimental group （P 〈 0.05）. CONCLUSION： EGB761 can obviously improve Na^＋-K^＋-ATPase and Ca^2＋-ATPase activities of disused atrophy skeletal muscle to effectively inhibit the occurrence of disused skeletal muscle atrophy.