实验性脾虚证大鼠睾丸形态学与细胞化学的研究

A Morphological and Cytochemical Study on Testes of Rats with Experimental "Spleen Deficiency" Syndrome

对实验性脾虚证和对照组大鼠的睾丸进行常规组织学与细胞化学的对比观察。结果显示：脾虚组睾丸体积明显缩小，曲细精管呈不规则状，管径缩小，管壁变薄，细胞间质水肿明显。细胞化学观察显示各级生精细胞中ＳＤＨ、ＡＬＰ、ＡＣＰ和ＡＴＰａｓｅ活性有明显变化，睾丸间质细胞３－β－ＨＳＤＨ活性有轻度增强。研究结果表明脾虚证不仅表现为消化系统功能低下，生殖系统也有病理学改变。

Twenty adult Wistar rats were used and divided into two groups,i.e.normal control group and experimental 'spleen deficiency' group.The experimental rat had been fed with the decoction of Dahuang.The animals of both groups were killed simultaneously 42 days la ter ,the testes of each animal were studied morphologically and cytochemically.The results of morphological observation in experimental 'spleen deficiency' group showed: ①The volume of testis had shrunk.②The seminiferous tubules had changed into irregular shape.③The diameter had shrunk.④The wall had become thinner.⑤The intercellular substances became edematous. The reactivity of SDH, ALP, ACP, ATPase of spermatogonic cells in experimental 'spleen deficiency' group showed definite changes on cytochemistry. The reactivity of 3 β HSDH in interstitial cells was weaker than normal control group. This study demonstrated not only that the function of digestive system became lowered,but also the reproductive system underwent pathological changes.