上海地区部分婴幼儿腹泻标本中诺如病毒基因分析

Gene sequence analysis of the Norovirus detected in diarrhea samples from infants in a region of Shanghai

目的了解2006年上海哨点医院婴幼儿疑似病毒性腹泻散发病例中诺如病毒基因型别和基因特征。方法应用反转录-聚合酶链反应(RT-PCR)扩增标本中诺如病毒核酸,测序后使用DNAstar基因分析软件与Genbank中的参考株进行核酸序列分析。结果13份测序结果均属于诺如病毒GⅡ遗传组,以GⅡ.4基因型为主。其中2份核酸序列与2006年德国汉堡毒株序列同源性相近,分别为86.2%和88.2%;2份核酸序列与2006年荷兰乌得勒支毒株序列同源性相近,均为83.9%;其余9份核酸序列与2004年中国广西、2004年和2005年日本东京毒株序列同源性相近,为87.3%～97.5%。结论2006年上海哨点医院婴幼儿疑似病毒性腹泻标本中存在诺如病毒GⅡ.4基因型散发病例,不同病毒株之间差异较大。

Objective In order to study the genotype and the genetic identity of Norovirus in sporadic infant diarrhea samples collected from surveillance hospitals from the Shanghai area in 2006.Methods The viral RNA was RT-PCR amplified using human caliciviruses-specific primers. The amplified genes were then sequenced and analyzed using Genetic Analyzer and DNAstar software to complete genotyping and homology analysis.Results Homology analysis revealed that the sequences of 13 specimens were homogeneous with Noroviras G 11 . The sequences of 2 specimens were closer to the viral sequence obtained from Hamburg in 2006. The sequences of 2 other specimens were closer to the sequences obtained from a virus from Utrecht in 2006 and the sequences of 9 other specimens were closer to those of sequences from Guangxi in 2006 and Tokyo between 21304 and 2005. Conclusion The cases of diarrhea presented at various surveillance hospitals in Shanghai were diverse with regard to Noroviras genogroup G Ⅱ The majority of the sporadic strains in this study belonged to genotype G Ⅱ .