葛根总黄酮的提取及抗氧化活性评价研究

Study on Extracting Process of Pueraria Flavone and Evaluation of Anti-oxidant Activity

以野葛根为原料,以醇回流法提取总黄酮,根据单因素及正交试验确定了最佳工艺条件,并对提取的葛根黄酮采用邻苯三酚-碳酸盐缓冲溶液-鲁米诺和鲁米诺-H2O2两种化学发光体系进行抗氧化活性评价。研究结果表明:最佳工艺条件为70%(V/V)的乙醇水溶液作为提取剂,浸提时间为1h,料液比为1:30(m/V),浸提温度为80℃。葛根黄酮清除O2·的IC50为28.94μg/ml,清除H2O2的IC50为46.30μg/ml。

The optimal process of extracting flavone from Pueraria by ethanol was studied and the optimal conditions were obtained by orthogonal matrix on the basis of single factor test in this paper. Meanwhile, the anti-oxidant activities of the extraction liquid were also studied by Pyrogallol-carbonate buffer-Luminol chemiluminescence system and Luminol-H2O2 chemilumines- cence system. The results showed that the optimal conditions by orthogonal matrix were in the following： time 1 hour, the temperature 80 ℃ the volume of ethanol 70% and the ratio of material to solvent 1：30. It was also found that the CL intensity of the Luminol - H2O2 system and Pyrogallol-carbonate buffer-Luminol system could be inhibited in the presence of flavonoids of pueraria. It was demonstrated that the flavonoe of Pueraria had powerful ability to clear off superoxide radicals （IC50=28.94 μg/ml） and hydrogen peroxide （IC50=6.30μg/ml）.