摘要
采用单因素和正交试验设计对茶叶中β-D-半乳糖苷酶的比活力测定条件进行优化。结果表明,茶叶中β-D-半乳糖苷酶1比活力测定条件为:以pH4.0的柠檬酸-磷酸氢二钠为缓冲液,在60℃下反应7min。对不同茶叶品种、生长季节以及不同嫩度叶片中的β-D-半乳糖苷酶比活力进行分析。结果表明,乌龙茶与绿茶品种中β-D-半乳糖苷酶比活力差异明显,不同季节与不同嫩度茶叶叶片之间也存在较大变化。
β-D-galactosidase was extracted from tea leaves. Single factor analysis and orthogonal experiment were adopted to obtain the optimal conditions for activity assay. The results showed that the activity ofβ-D-galactosidase can be greatest if it is investigated at 60 ℃ for 7 min with citrate-phosphate as buffer （pH4.0）. The difference of β-D-galactosidase activities in tea species, seasons and tenderness were analyzed. It was found that there are obvious different activities between oolong tea and green tea species. At the same time, the activity diversity also exists in various seasons and leaf tenderness.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第10期384-388,共5页
Food Science
基金
国家自然科学基金资助项目(30270939)
