摘要
详细报道了应用SOS显色反应评价巴马长寿老人源乳杆菌和双歧杆菌抑制基因毒性物质4-硝基喹啉-1-氧化物(4-NQO)的方法。4-NQO(0.1mmol/L)与待测乳酸菌(108~109CFU/ml)37℃共培养180min,6株唾液乳杆菌和2双歧杆菌培养上清液均可抑制4-NQO的基因毒性作用,不同菌株之间在抑制能力上存在菌株差异性。其中以分离自长寿老人粪便的Lactobacillus salivarius subsp.salivarius24DB、2JC、BCHONG和Bifidobacterium animalis subsp.lactis BBMN BBMN1、BBMN2的基因毒性清除率分别为92.91%、88.55%、89.81%、80.86%和83.54%,初步讨论了益生菌对4-NQO的抑制机理。
Antigenotoxic activity against 4-nitroquinoline-1-oxide (4-NQO)of Bfidobacterium and Lactobacillus isolated from faecal sample of elderly people was studied using the shortterm bacterial assay SOS-chromotest, with Escherichia coli PQ37(sfiA:: lacZ) as the tester organism. It was found that the genotoxicity of 0.1 mmol/L 4-NQO was reduced after coincubation with probiotics (108~109 CFU/ml). All isolates showed potential for deactivating of 4-NQO, and the inhibitory ability was strain specific. Lactobacillus salivarius 24DB 2JC, BCHONG, Bifidobacterium animalis subsp. lactis BBMN BBMN1 and BBMN2 has the higher antigenotoxic ability as 92.91%, 88.55%,89.81%, 80.86% and 83.54% respectively. The possible mechanism was discussed primely.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第10期425-429,共5页
Food Science