青钱柳多糖对人宫颈癌HeLa细胞和人脐带内皮细胞生长的影响

Effects of Polysaccharide from Cyclocarya paliurus (Batal.) Iljinskjk on Growth of HeLa Cells and Human Umbilical Vein Endothelial Cells

目的:研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人宫颈癌HeLa细胞和人脐带内皮细胞(HUVEC)生长的影响。方法:采用噻唑蓝(MTT)法,观察不同浓度、不同提取方法获得的PCP对HeLa细胞和HUVEC细胞生长的影响。结果:PCP I、II在50、100、200、400μg/ml浓度条件下,均可极显著性抑制HeLa细胞生长(p<0.01)。PCP I、II对HUVEC细胞生长有一定影响,但增殖抑制率不高;在PCP抑癌作用最高的浓度处(200μg/ml),PCP对HUVEC细胞的生长没有影响。结论:PCP极显著性抑制HeLa细胞的生长;在P C P抑癌作用最高的浓度处,P C P对H U V E C细胞的生长没有影响。

Objective： To investigate the regulate effects of Polysaccharides isolated from Cyclocarya paliurus （Batal.） Iljinskjk （PCP）on human cervical carcinoma HeLa cells and human umbilical vein endothelial cells（HUVEC） in vitro. Methods： MTT method was used to observe the effects of PCP, which were acquired by different extraction methods, on the HeLa and HUVEC cells respectively at different concentration. Results： PCP Ⅰ and Ⅱ can both inhibit the proliferation of HeLa cells extremely （p〈0.01） at the concentration of 50, 100, 200, 400μg/ml. PCP Ⅰ and Ⅱ can influence the growth of HUVEC cells, but Inhibition of cell proliferation was not sharp; PCP can＇t influence the growth of HUVEC cells at the concentration which the inhibitory rate of PCP is the most distinct on HELA cells （200μg/ml）. Conclusions： PCP can significantly inhibit the proliferation of HeLa cells, and it can＇t influence the growth of HUVEC cells at the concentration which the inhibitory rate of PCP is the highest on HeLa cells.