摘要
目的:建立荷移光谱法测定双氯芬酸钠(DS)的含量。方法:分别以7,7,8,8-四氰基对二次甲基苯醌(TCNQ)在丙酮介质中和氯冉酸(CL)在乙醇介质中为荷移试剂,在350~900nm 波长处扫描吸收光谱。结果:DS 与 TCNQ 形成的络合物在743nm 和842nm 有2个吸收峰,表观摩尔吸光系数分别是2.10×10~4L·mol^(-1)·cm^(-1)和3.07×10~4L·mol^(-1)·cm^(-1);与 CL形成的络合物在530nm 处有吸收峰,表观摩尔吸光系数8.77×10~2L·mol^(-1)·cm^(-1)。药物浓度分别在3~50mg·L^(-1)(TCNQ法)和15~750mg·L^(-1)(CL 法)范围内服从比尔定律,平均回收率分别为97.8%和98.6%,RSD 分别为1.4%,1.0%(n=6)。用摩尔比法和等摩尔连续变化法测得荷移络合物中 DS 与配体的摩尔比均为1:1。结论:本方法操作简便,结果准确可靠,适用于测定 DS 片中的含量。
Objective:To establish an method of charge transfer spectrophotometry for determination of diclofenac sodium base (DS). Method:Scan absorption spectra ranging from 350 nm to 900 nm by the charge transfer reagent of 7,7,8,8 - tetracyanoquinodimethane (TCNQ) in acetone and chloranilic acid (CL) in ethanol, respectively. Resuits:The apparent molar absorptivity of TCNQ complex was 2. 10 × 10^4 L · mol^-1 · cm^-1at 743 nm,and 3.07 × 10^4 L · mo1^-1 · cm^-1at 842 at;That of CL complex was 8.77 × 10^2 L · mo1^-1 · cm^-1. Beers law obeyed in the concentration range of 3 - 50 mg · L^-1 for TCNQ method and 15 - 750 mg · L^-1 for CL method. The average recoveries were 97. 8% and 98.6%. The relative standard deviations of the two methods were 1.4% and 1.0 % ( n = 6) ,respectively. The composition of complex of DS with TCNQ or CL was 1:1. Conclusion:This method is simple, rapid and reliable for determination of DS in tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第10期1654-1657,共4页
Chinese Journal of Pharmaceutical Analysis