高效液相色谱法测定复方苦参素胶囊中苦参碱的含量

HPLC determination of matrine in compound Kushensu capsules

目的:建立高效液相色谱法测定复方苦参素胶囊中苦参碱的含量。方法:以 Hypersil C_8色谱柱(4.6mm×150mm,5μm)为色谱柱,柱温25℃,用甲醇-水-三乙胺(45:55:0.02)为流动相,流速0.8mL·min^(-1)。进样量10μL,于220nm 波长处,以外标法测定。结果:苦参碱在10.7～161.0μg·mL^(-1)浓度范围内线性关系良好(r=0.9999)。平均回收率为99.6%。结论:本方法灵敏、简便、准确。

Objective：To establish an HPLC method for the determination of matrine in compound Kushensu. capsules. Methods：By HPLC using as Hypersil Cs column（4. 6 mm × 150 mm,5 μm ）and the mobile phase methanol- water - trietylamine （45： 55： 0. 02）, at a flow of 0. 8 mL · min^-1, and the UV detection at 220 nm. The column was 25 ℃ and the injection volume was 10 μL. Results：The method was linear over the ragne of 10. 7 - 161.0 μg · mL^-1 （r =0. 9999）. The average recovery was 99. 6%. Conclusion ：The method is sensitive ,simple and accurate.