低温保存大鼠同种异体主动脉与肺动脉移植后钙化的对比

Comparative investigation of the calcification of the cryopreserved aortic and pulmonary homografts after implantation

目的：定量研究低温保存的同种异体主动脉与肺动脉移植后的钙化．方法：采用同种异体主动脉和肺动脉管道异位植入大鼠腹主动脉的移植模型．移植８周后取出移植物应用微电子探针分析技术测定主、肺动脉（干重）含钙量．结果：移植前主动脉的含钙量（０．２４±０．０５）μｇ／ｍｇ明显高于肺动脉的含钙量（０．１３±０．０２）μｇ／ｍｇ，Ｐ＜０．０１；移植后主动脉含钙量（０．５３±０．０９）μｇ／ｍｇ明显高于移植前（０．２４±０．０５）μｇ／ｍｇ，含钙量上升１２３．４％±５３．０％，Ｐ＜０．０１；移植后肺动脉含钙量（０．２１±０．０３）μｇ／ｍｇ明显高于移植前（０．１３±０．０２）μｇ／ｍｇ，含钙量上升６３．８％±３５．８％，Ｐ＜０．０１；移植后主动脉含钙量（０．５３±０．０９）μｇ／ｍｇ明显高于肺动脉含钙量（０．２１±０．０３）μｇ／ｍｇ，Ｐ＜０．０１．结论：在同种异体主动脉和肺动脉的移植过程中主动脉比肺动脉更容易钙化．微电子探针技术在生物标本元素组成的分析中具有重要的价值．

Objective: This experiment was performed to quantitively investigate the calcification of the cryopreserved aortic and pulmonary homografts after implantation. Methods: In operation the heterotopic implantation of homograft conduits into the abdominal aorta was adopted. The calcification of the homograft conduits was determined by SX micro electronic probe analysis 8 weeks after implantation. Results: Before implantation the calcium content of aortic homografts (0.24±0.05) μg/mg was significantly higher than that of pulmonary homograft (0.13±0.02) μg/mg, P <0.01. After implantation the calcium content of arotic homografts (0.53±0.09) μg/mg was significantly higher than that before implantation (0.24±0.05) μg/mg, with an increase by (123.4±53.0)%, P <0.01, and so was that of pulmonary homografts [from (0.13± 0.02 ) μg/mg to (0.21±0.03) μg/mg], with an increase by (63.8±35.8)%, P <0.01. And the calcium content of aortic homograft (0.53±0.09) μg/mg was still significantly higher than that of pulmonary homograft (0.21±0.03) μg/mg, P <0.01. Conclusion: In homograft conduit implantation the aortic homografts are more easily calcified than pulmonary homografts. The micro electronic probe technique has a great significance in element component analysis of biological preparation.