不同神经移植体修复神经缺损对轴浆逆行运输和胞体的影响

The influence of neurologic defect repair with the different structure neural transplant on axoplasm retrogression transport and cell body

目的研究不同结构神经移植体修复神经缺损后对轴浆逆行运输和胞体的影响。方法新西兰兔18只,显露和游离双侧耳大神经,各造成1.2cm长缺损;动物随机分为3组:A组,耳大神经移植组;B组,隐神经移植组;C组,股外侧皮神经移植组,修复方法采用外膜缝合。术后12周HRP逆行标记,各组于注射HRP后24、36、48h分别取2只行TMB显色,Image Pro Plus图像分析软件对标本进行图像分析。结果B组轴浆逆行运输速度大于A组和C组,B组阳性胞体数量大于A组和C组,A组大于C组,B组阳性胞体直径大于A组和C组,组间差异具有显著性。结论选用轴突密度大、轴突数量多的神经移植体能够促进轴浆逆行运输的恢复。

Objective To investigate the impact of neurologie defect repair with the different structure neural transplant on axoplasm retrogression transport and cell body.Method Authors divided 18 New Zealand rabbits, for which first acting out and dissociating their biateral aurieularis magnus with the artificial lesions long 1.2em had taken, random into three groups： A, transplanted with the aurieularis magnus; B, transplanted with the saphenous nerve; C, transplanted with the lateral femoral cutaneous nerve; then them all recieved repairing the lesion with the adventitia saturation technique.Twelve weeks after the operation,retrogression tracing the nerves with the HRP was taken.After the HRP injection, eolourating with the TMB at time points of 24h,36h,48h,then the images with the Image Pro Plus Software were analysed.Results The axoplasmic transport velocity of group B was quicker than that of group A and group C, the positive number of cell body in group B was bigger than in group A and group C （P〈0.05）, and these in group A was also bigger than group C（P〈0.05）. The diameter of positive cell body in group C was significantly longer than in group A and group C（P〈0.05）, the differences was statistically significant. Conclusion The neural transplant with the higher neuraxon density and nmre neuraxons can promote the recovery of axoplasm retrogression transport.