摘要
目的:通过研究芪丹通脉片对血清中细胞因子水平的影响,探讨芪丹通脉片对大鼠心肌缺血再灌注损伤的保护机制。方法:采用线栓法制备大鼠心肌缺血再灌注模型,选用SD大鼠36只,随机分为6组(n=6),即假手术组、模型组、芪丹通脉片低剂量组、芪丹通脉片中剂量组、芪丹通脉片高剂量组、恬尔心组,各组均用生理盐水配制等体积药液灌胃14d,每日1次。造成大鼠心肌缺血再灌注模型,采用双抗体夹心ABC-EL ISA法检测各组大鼠血清中肿瘤坏死因子-α(TNF-α),白介素-6(IL-6)含量。结果:芪丹通脉片可有效降低大鼠心肌缺血再灌注后血清中TNFα-、IL-6的含量。结论:芪丹通脉片对心肌缺血再灌注损伤有明显的保护作用,减轻病灶处白细胞的聚集。这种保护作用部分与其降低血细胞因子水平有关,从而为其临床应用提供理论依据。
Objective：To observe the effects of Qidantongmai tablet on serumal cytokines in Rat during Ischemia and Reperfusion Injury of Heart and the possible action mechanism. Methods ： 36 male SD rats were randomly divided into 6 groups： model group; Control group; QDTMT low dose group; QDTMT medium dose group; ODTMT high dose group, Tianerxin group （n = 6）. Different drug or normal saline was administered to the 6 groups at the same dosage once a day for 14 days before operation. Then myocardial ischemia reperfusion models were established and the whole bloods of all rats in each groups were collected and then centrifuged to determine the content of serumal TNF-α and IL-6. Results ： The concentration of TNF-α and IL-6 were remarkably decreased and there was both obvious significance in two treatment groups compared with model group. Conclusion： The results demonstrate that Qidantongmai tablet provides similar remarkable protective effects in rat model of iscbemia reperfusion and the protective effect of Qidantongmai tablet is partially due to reducing the concentration of TNF-α and IL- 6.
出处
《陕西医学杂志》
CAS
北大核心
2007年第6期645-646,659,共3页
Shaanxi Medical Journal
基金
全军新药开发课题(后卫中医字第3号)
