高碘饮食对不同鼠种甲状腺球蛋白的储存及含量影响的形态计量学研究

The morphometry analysis of iodine excess on the store and content of thyroid globulin in rats and mice

目的研究高碘饮食对不同鼠种(大鼠和小鼠)甲状腺球蛋白的储存及含量的影响。方法应用MIAS-2000型图象分析系统,选用甲状腺滤泡腔截面积、胶质面积与视场面积比值(面积比)、胶质平均灰度3项指标,对实验12周Wistar大鼠及Balb/c小鼠的甲状腺组织切片进行形态计量学测量及统计分析。结果随着碘摄入量的增加,大鼠各高碘组的甲状腺滤泡腔截面积和面积比,呈升高趋势,而胶质平均灰度却明显低于对照组(P<0.05或P<0.01)。小鼠各高碘组随着碘摄入量的增加,甲状腺滤泡腔截面积、面积比、胶质平均灰度均呈升高趋势。结论小鼠随着碘摄入量的增加,滤泡腔逐渐增大,甲状腺球蛋白含量明显增多,甲状腺发生了大滤泡胶质蓄积性肿大;而大鼠未形成甲状腺肿大,甲状腺球蛋白含量也不见明显改变,表明大鼠对高碘有比较强的耐受能力。

Objective To study the effect of iodine excess on the store and content of thyroid globulin in rats and mice. Methods The morphometry analysis was adopted on thyroid histological sections of Wistar rats and Balb/c mice at 12 weeks of experiment. The sectional area of thyroid follicular cavity, the area ratios of colloid and field view, and the average gray density of colloid were measured by MIAS-2000 Image Analysis System. Results In rats, the area of thyroid follicular cavity and the area ratios were gradually increased from NI to 50HI group. The gray density in iodine excess groups was lower than that in NI group（P 〈0.05）. Whereas in mice, above three indexes in all iodine excess groups were obviously increased with the iodine intake increasing. Conclusions In iodine excess, the large follicle and colloid accumulative goiter occurred in mice with manifestations of enlarged follicular cavity and increased colloid contents. But the goiter was not found in rats, which indicated that rats may have stronger tolerance to iodine excess.