好氧反硝化细菌的筛选鉴定及处理硝酸盐废水的研究

Isolation and Identification of Aerobic Denitrifiers and Dispose the Wastewater of NO_3^--N

采用污泥驯化手段富集好氧反硝化细菌,将得到的驯化污泥分离纯化,共得到5株好氧反硝化细菌.f1f、2f、3f、5和f7的TN去除率为90.4%、91.2%、94.6%、95.6%和97%,表现出较好地去除总氮的能力.经过生理生化鉴定和16S rDNA测序,建立了系统发育树,可基本确定分离的菌株f1f、3和f5为Pseudomonassp.,分离菌f2和f4为Paracoccussp..采用筛选的5株好氧反硝化细菌建立连续流反应器.在反应器进入稳定运行阶段时,可以观察到系统对于硝酸盐的去除率稳定在98.16%左右.表现出较好的硝酸盐去除效果,出水亚硝酸盐含量一直维持在较低的水平,其最大值不超过3.56 mg/L.COD的平均去除率为87.24%,基本实现了同一反应器中有机物和硝酸盐的共同去除.

After domesticating activated sludge to enriched aerobic denitrifiers,5 aerobic denitrifiers were isolated from it.The removal rates of TN were 90.4%, 91.2% ,94.6% ,95.6% and 97% by fl, f2, t3, t5 and t7, respectively. According to the physiological biochemical index, five strains of aerobic denitrifiers were generally identified as Pseudomonas sp., Paracoccus sp., respectively. The establishment of continuous flow reactor surveyed various indices. And the efficiency of nitrate nitrogen removal can reach up to 98.16%, when the reactor works smoothly. The nitrite concentration of effluent was at a low level and the maximum value was no more than 3.56 mg/L. At the same time, the efficiency of COD removal was 87.24%, which demonstrates the removing of both organic and nitrate nitrogen