HIF-1α及其调控基因PTEN在胃癌中表达的相互关系及临床病理意义

Expression and significance of hypoxia-inducible factor-1α and its regulator phosphatase and tensin homolog deleted in chromosome 10 in gastric cancer

目的:探讨缺氧诱导因子-1α(HIF-1α)及其调控基因PTEN在胃癌及正常胃组织中表达的相互关系以及他们在胃癌的发生发展、浸润和转移中的作用.方法:应用免疫组化SP法检测54例胃癌组织中HIF-1α和PTEN的表达情况,探讨他们与胃癌临床病理因素的关系.结果:HIF-1α在胃癌中的表达率(74.07%)明显高于正常胃组织(0%)(P<0.01);HIF-1α的表达与胃癌TNM分期(P<0.05)、肿瘤浸润深度(P<0.01)和淋巴结转移显著相关(P<0.05).胃癌中PTEN低表达(51.9%),且在肿瘤浸润深(38.5%vs 86.7%,P<0.01)、有淋巴(28.0%vs 72.4%,P<0.01)和远隔转移(16.7%vs 61.9%,P<0.05)、临床分期高(28.6%vs 76.9%,P<0.01)、病理分化低(22.2%vs 70.6%,63.2%,P<0.01)的胃癌组织表达明显降低.胃癌组织中HIF-1α与PTEN的表达呈负相关(r=-0.41,P<0.05).结论:HIF-1α和PTEN在胃癌的发生发展中起重要作用,联合检测可作为判断胃癌恶性程度和预后的指标.

AiM： To study the expression of hypoxiainducible factor-1α （HIF-1α） and its regulator phosphatase and tensin homolog deleted in chromosome 10 （PTEN） in gastric cancer and their relationship with tumor angiogenesis and clinicopathologic features. METHODS： Immunohistochemistry was performed to detect the expression of HIF-1α and its regulator PTEN in 54 cases of gastric cancer. The relationships among the expression of HIF- 1α and PTEN, tumor angiogenesis and clinicopathologic features of patients were analyzed. RESULTS： The positive expression rate of HIF- 1α in gastric cancer （74.07%） was significantly higher than that in normal gastric tissue （0%,P 〈 0.01）; the expression of HIF-1α in gastric cancer was significantly associated with TNM stage （P 〈 0.05）, invasive depth （P 〈 0.01） and lymph-node metastasis （P 〈 0.05）; the positive expression rate of PTEN in gastric cancers was low （51.9%）, and reduced with increased invasive depth （38.5% vs 86.7%, P 〈 0.01） and clinical stage （28.6% vs 76.9%, P 〈 0.01）, reduced histological differentiation （22.2% vs 70.6%, 63.2%, P 〈 0.01） and the occurrence of lymphatic （28.0% vs 72.4%, P 〈 0.01） and long distance metastases （16.7% vs 61.9%, P 〈 0.05）. There was a negative correlation between the expression of HIF-1α and PFEN （r = -0.41, P 〈 0.05）. CONCLUSION： HIF-1α and its regulator PTEN play a very important role in the development of gastric cancer and could be useful factors in the diagnosis of gastric cancer and estimation of prognosis.