GMC0403注射液对氰化钠诱导原代培养心肌细胞损伤的保护作用

Protective effects of GMC0403 injection on primary culture of rat cardiac myocyte injury induced by NaCN in vitro

目的研究GMC0403注射液对氰化钠诱导原代培养心肌细胞损伤的保护作用。方法采用1μmol/ml氰化钠与低糖DMEM、共培养复制心肌细胞缺糖、缺氧损伤模型,通过细胞线粒体琥珀酸脱氢酶活力(MSDH)、乳酸脱氢酶(LDH)外漏率、台盼蓝染色率研究GMC0403注射液对原代培养心肌细胞氰化钠(NaCN)诱导缺糖、缺氧模型的保护作用。结果GMC0403注射液能显著降低缺糖、缺氧损伤心肌细胞线粒体琥珀酸脱氢酶活力,抑制LDH的外漏,降低台盼蓝染色率。结论GMC0403注射液对NaCN诱导原代培养心肌细胞损伤具有保护作用。

Objective： To investigate protective effects of GMC0403 injection on primary culture of rat cardiac myocyte injury induced by NaCN in vitro. Methods：After adding GMC0403 injection, the primary culture of rat cardiac myocyte was injured by 1μmol/ml NaCN and low glucose DMEM. The release ratio of lactic dehydrogenase （LDH） from cardiac myocyte, mitocbondrion succinic dehydrogenase （MSDH）, and staining ratio by trypan-blue were assayed. Results： GMC0403 injection significantly increased the MSDH activity （volume of A570 significantly increasing）, decreased the release ratio and staining ratio of trypan-blue. Conclusion： GMC0403 injection could ameliorate the primary culture of cardiac myocyte induced by NaCN.