U83 Box C/D snoRNA构建果蝇科系统发生树

U83 box C/D snoRNA as a Molecular Marker in the Phylogeny of Drosophilidae

利用已报道的黑腹果蝇U83基因搜索果蝇基因数据库,鉴定了10种新的果蝇科U83同源基因,它们均位于相应物种核蛋白基因rpl3的内含子中。以冈比亚按蚊为外类群,对11种果蝇的U83核苷酸序列作进化关系分析,用邻接法重建了系统发生树,结果与传统方法构建的系统发生树相比,能反映果蝇科的大致进化关系,但还存在部分差别。为增加序列信息,把序列长度拓展至整个U83所在的内含子,同法构建系统发生树,结果与传统系统发生树几乎完全一致。该研究是用boxC/D snoRNA基因序列构建系统发生树的首次尝试,实验结果证明U83可以很好地用于构建果蝇科内各物种的种系发生树。

Based on FlyBase and U83 in Drosophila melanogaster, ten new U83 gene homologues were identified in Drosophilidae which were located in intron of rpl3 respectively. The homologous sequences were compared, and the molecular trees were reconstructed by neighbor-joining method of PAUP4.0 program using Anopheles gambiae as outgroup. The result reflected the evolutionary relationship in Drosophilidae roughly. Se- quences were enlarged to the entire U83 located introns to get more information, and the phylogenetic tree con- structed by those sequences was more correct compared with the former one. Genes of box C/D snoRNA were used for constructing phylogenetic tree for the first time, our study confirmed the good effect of U83 box C/D snoRNA as a molecular marker in the phylogenetic analysis of Drosophilidae.