细胞凋亡检测方法探讨

Investigate on the Methodology of Detecting Apoptosis

采用图例法和比较法分析评价细胞凋亡检测方法的应用范围和检测结果的相关性,指出只有选择多种适当的方法进行综合检测,才能对结果做出正确而合理的分析判断。流式细胞术简便、快速,从膜上分子、胞内蛋白到DNA水平全方位多角度分析凋亡,可作为检测细胞凋亡的首选方法。

By the methods of graphical presentation and comparison, we analyzed and evaluated the relevance between the results got by different apoptosis-detecting techniques and its applicability. Then we found that the more right and reasonable judgments relay on the combination using of different appropriate apoptosis- detecting techniques. Among these methods, flow cytometry has its own advantages. It is more convenient and faster to detect much apoptosis features including membrane moleculars, intracytoplasmic proteins, and DNA fragments. Thus, flow cytometry is recommended to be used as the first choice for apoptosis-detecting.