吸烟对脑梗死患者尿液中8-异前列腺素F_(2α)含量的影响

Impact of smoking on urinary 8-iso prostaglandin (PG) F_(2α) level in patients with cerebral infarction

目的探讨吸烟对脑梗死患者尿液中8-异前列腺素F2α(8-iso-PGF2α)含量的影响。方法选取急性脑梗死患者28例,其中14例(有吸烟史)作为吸烟组,另外14例(无吸烟史)作为对照组,两组的年龄、性别、血压、血脂、血糖、病灶部位及脑梗死程度无显著性差异。用酶联免疫分析法测定所有受试者尿液中8-iso-PGF2α的浓度及空腹静脉血中低密度脂蛋白(LDL)总胆固醇、LDL三酰甘油、LDL游离胆固醇水平。结果吸烟组脑梗死患者尿液中8-iso-PGF2α的浓度明显高于对照组(75.79±10.76vs67.36±9.18ng/mmol creatinine,P<0.05)。静脉血中LDL总胆固醇、LDL三酰甘油、LDL游离胆固醇在吸烟组与对照组间无显著性差异(分别为5.12±0.61vs4.73±0.62mmol/L,0.89±0.07vs0.85±0.04mmol/L,1.71±0.43vs1.74±0.91mmol/L,P>0.05)。结论吸烟可以使脑梗死患者尿液中8-iso-PGF2α浓度增高,增加患者体内的氧化压力。

Objective To investigate the impact of smoking on the level of urinary 8-iso prostaglandin （PG） F2α （8-iso-PGF2α） of patients with acute cerebral infarction. 8-iso-PGF2α is a product of arachidonic acid catalyzed by free-radical, and it has been identified as an indicator of oxidative stress in vivo during reperfusion. Methods Plasma and urine samples were obtained from 28 patients with acute cerebral infarction. Among them 14 patients with no smoking habit were chosen as control group according to the selection criterion, and the other 14 patients with smoking habit were chosen as investigation group according to the same criterion. There was no significant difference between the two groups in age, gender ratio, blood pressure, lipids level, blood glucose, focus location, and degree of the cerebral focus of infaration. The concentration of urinary 8-iso PGE2α and levels of low-density lipoproteins （LDL）, total cholesterol, LDL-triglyceride （TG） and LDL-free cholesterol in plasma were measured by enzyme immunoassay. The quantification of urinary 8-iso PGF2α and LDL levels in plasma were performed for each sample respectively. Results The concentration of urinary 8-epi PGF2α of the patients with smoking habit was significantly higher than that in the patients of control group （75. 79±10. 76 vs 67. 36±9. 18 ng/mmol creatinine, P〈0.05）. There was no significant difference in LDL total cholesterol, LDL-TG and LDL-free cholesterol （5. 12±0. 61 vs 4. 73±0. 62 mmol/L, 0. 89± 0.07 vs 0. 85±0. 04 mmol/L and 1. 71±0. 43 vs 1. 74±0.91 mmol/L, P〉0.05, respectively） between the two groups. Conclusions Cigarette smoking may raise the urinary 8-iso-PGF2α level of patients with acute cerebral infarction, suggesting an increase in the level of oxidative stress in vivo in these subjects.