树突状细胞β-中心体肌动蛋白表达与肝癌侵袭转移能力相关

Expression of β-centraction in dentritic cells is associated with invasive and metastatic capacity of hepatocellular carcinoma

目的研究β-中心体肌动蛋白（β-centractin）与肝细胞癌病人侵袭、转移能力之间的关系。方法分离26例肝细胞癌病人外周血单个核细胞，体外培养加入细胞因子诱导生成树突状细胞（DC）。收集同一病人肝癌切除标本，制备肿瘤细胞裂解物负载自体DC。Western Blot检测DC中β-centractin的表达，分析其与肝癌临床病理特性之间的关系。结果负载自体肿瘤细胞裂解物DC β-centractin表达水平，在肿瘤包膜完整和无癌栓组分别高于包膜不完整和有癌栓组，与有无子灶、AFP水平及肿瘤大小无关。结论负载自体肿瘤细胞裂解物DC β-centractin表达水平与肿瘤侵袭转移能力呈负相关。

Objective To explore the relation of expression of β-centraction in dentritic cells （DCs） from patients with hepatocellular carcinoma （HCC） to invasive/metastatic capacity of HCC. Methods Peripheral blood monocytes isolated from 26 patients with HCC were used for subsequent induction of DCs. Operative specimens of the same group of patients were simultaneously collected and prepared for tumor cell lysates. Then DCs were pulsed with auto-tumor lysates and the expression of β-centraction was determined by Western blotting. Results The level of β-centraction in DCs pulsed with lysates in HCC with tumor encapsulation and/or without tumor thrombi was significantly higher than that in HCC without encapsulation and/or with tumor thrombi. However, the expression of β- centraction was not correlated with the spreading nodules, AFP level and tumor size. Conclusions The expression of β-centraction in DCs is associated with the invasive and metastatic capacity of HCC.