肝癌及癌旁组织中β-葡萄糖醛酸酶蛋白及mRNA的表达及意义

Expression and significance of β-glucuronidase and its mRNA in hepatocellular carcinoma and paracancerous tissue

目的探讨肝细胞癌及癌旁组织中β-葡萄糖醛酸酶（β-G）蛋白及β-G mRNA的表达和临床意义。方法采用逆转录-聚合酶链反应（RT-PCR）方法和免疫组织化学方法，对25例原发性肝细胞癌及其癌旁组织和10例正常肝脏组织进行检测，并分析其与临床病理特点之间的关系。结果半定量分析显示β-G mRNA的表达在肝脏癌组织（3．71±0．32）与癌旁肝组织（1.83±0.22）以及正常肝脏组织（1．71±0．32）间差异比较，均有统计学意义（均为P〈0．01），而癌旁肝组织和正常肝组织间比较，差异无统计学意义（P〉0．05）；从正常肝组织、癌旁组织到肝癌组织β-G蛋白的阳性细胞表达率分别为（48．2％±5．5％）、（56．8％±6．2％）和（83．8％±9．1％），呈递增趋势（χ^2=42．33，P〈0．01）；β-G蛋白的表达与肝癌病人的门脉癌栓以及淋巴结转移等临床因素有关（P〈0．01），而与肿瘤大小、AFP水平无关。结论β-G与肝细胞的癌变过程密切相关，可能在肝癌的侵袭和转移过程中也起一定的作用。

Objective To investigate the expression of β-glucuronidase （β-G） and its mRNA in hepatocellular carcinoma （HCC） and paracancerous tissue and explore its significance. Methods RT- PCR and immunohistochemistry were used to detect the β-G expression and its mRNA in 25 patients with HCC and 10 healthy individuals. The relationship between β-G and clinicpathological features of HCC was determined. Results Semiquantitative analysis showed that the expression of β-G mRNA was significantly different among the HCC, its corresponding paracancerous tissue and normal liver tis sue （P〈0. 01）. There was no marked difference in the expression between the paracancerous tissue and normal liver tissue （P〉0. 05）. The expression rate of β-G was 48. 2±5.50%, 56. 80%±6.20% and 83.8%± 9.1% in the normal liver tissue, paracancerous tissue and HCC, respectively （P〈0. 01）. The expression of β-G was significantly correlated to tumor thrombi in portal vein and lymph node metastasis of HCC （P〈0. 01） but not to tumor size and AFP level （P〉0. 05）. Conclusions β-G is closely correlated with carcinogenic change of hepatic cells and may play an important role in progression and metastasis of HCC.