染料介导光氧化法处理的同种异体动脉体内近期抗钙化性能评价

Anti-calcification function property of allogenic arteries cross-linked by dye-mediated photooxidation

目的观察染料介导光氧化法(DMP)处理的家兔胸主动脉用于同种异体腹主动脉置换后,在体内血流环境下的抗钙化性能。方法以经染料介导光氧化反应处理的家兔胸主动脉为研究对象,以戊二醛(GA)处理的家兔胸主动脉以及自体腹主动脉原位移植(OT)作为对照,建立家兔胸-腹主动脉移植模型;实验动物观察饲养1个月后,取出标本,通过原子吸收光谱法测定血管组织钙含量,扫描电镜观察血管壁钙化情况。结果DMP组血管壁钙含量为(3.58±0.22)μg/mg,GA组血管壁钙含量为(22.5±0.53)μg/mg,自体动脉移植组组织钙含量(2.99±0.33)μg/mg,DMP组组织钙含量虽较OT组高,但差异无统计学意义(P>0.05),DMP组组织钙含量较GA组低(P<0.01);扫描电镜下观察可见,GA组有大量的钙沉积,DMP组仅见少量钙沉积。结论光氧化反应处理的家兔胸主动脉在家兔胸-腹主动脉移植的动物模型中,抗钙化性能优于戊二醛固定的胸主动脉。

Objective： To study the anti-calcification function property of rabbit thoracic aorta cross-linked by dyemediated photo-oxidation. Methods Rabbits whose allogenic thoracic aorta was cross-linked by dye-mediated photo-oxidation （DMP, n =6） were in the DMP group, rabbits whose aUogenic thoracic aorta was cross-linked by glutaraldehyde （GA, n = 6） were in the GA group, and rabbits whose homogenic abdominal aorta was orthotropicaUy transplanted （OT, n = 6） were in the OT group. Then the donor arteries were transplanted into the position of the abdominal arteries of rabbits. After the operations, animals were fed for 1 month, and then the grafted artery was removed for observation by HE, SEM and the tissue calcium content which was analyzed by a flame atomic absorption spectrophotometer. Results： Calcium content in the DMP group, the GA group and the OT group was 3.58±0.22 μg/mg, 22.5 ± 0.53 μg/mg and 2.99± 0.33 μg/mg, respectively. There was no statistical difference between the DMP group and the OT group （ P 〉 0.05）. However, a significant difference between the DMP group and the GA group was revealed （ P 〈 0.01 ）. The calcium content in the DMP group was much lower than that in the GA group. Conclusion Arteries cress-linked by dye-mediated photo-oxidation treatment appears to have less calcification in the rabbit aUogenic transplantation model.