PTEN基因在小鼠胚胎着床过程中作用的实验研究

PTEN gene expression in mouse endometria increases during embryo implantation

目的探讨PTEN基因小鼠胚胎着床过程中的作用。方法实时荧光定量聚合酶链反应(real-time fluores-cence quantitativePCR,FQ-PCR)分别检测未孕及孕1、3、4、5、7d(分别记为d0、d1、d3、d4、d5、d7)小鼠子宫内膜PTEN基因mRNA的表达和子宫角注射反义寡核苷酸观察胚泡着床数。结果FQ-PCR测得妊娠的子宫内膜组织PTEN的表达高于未妊娠的子宫内膜组织,且随着妊娠天数的增加呈现逐渐增加的趋势,到妊娠第5天达到最高。子宫角注射PTEN反义寡核苷酸后胚泡着床数明显减少。结论PTEN在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。

Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation. Methods Real-time fluorescence quantitative PCR （FQ-PCR） was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice （day 1, day 3, day 4, day 5 and day 7）, with 20 mice sacrificed at each fixed day. Out of another 20 3-day pregnant mice, ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8. Results The PTEN mRNA/βactin mRNA in gradually hoisted as days passed by, and reached the the mice that received PTEN antisense oligonucleotide pregnant mice was higher than that of nonpregnant mice, highest at pregnant day 5. The number of blastocysts in was fewer than that received normal saline. Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.