急性心肌梗死大鼠心肌乙酰胆碱酯酶活性的变化

Changes in the heart acetylcholinesterase activities responsible for acute myocardial infarction in rats

目的:观察大鼠急性心肌梗死后心肌中乙酰胆碱酯酶活性的变化。方法:实验于2004-10/2005-04在哈尔滨医科大学附属第一医院中心实验室完成。①实验方法:选择雄性Wistar大鼠68只,采用结扎左冠状动脉前降支的方法制备大鼠心肌梗死及假手术(穿线后不结扎冠状动脉)模型。②实验分组:将术后存活的53只心肌梗死大鼠按随机数字表法分为心肌梗死后3,7,30d组各9,10,10只,将假手术大鼠24只做为各组的对照组,每组8只。③实验评估:从各组大鼠梗死中心、梗死周边、室间隔和右室取材,检测不同部位心肌中乙酰胆碱酯酶活性的变化。结果:存活的53只大鼠均进入结果分析。①心肌梗死后3d时,梗死中心区、梗死周边和室间隔中乙酰胆碱酯酶活性明显高于假手术组[分别为(14.12±3.55),(1.50±0.75)μkat/g;(16.59±7.17),(0.92±0.22)μkat/g;(8.64±4.02),(2.43±1.63)μkat/g],差异有显著性意义(t=3.168,3.367,P<0.01,t=2.817,P<0.05)。②心肌梗死后7d时,梗死中心区、梗死周边心肌中乙酰胆碱酯酶活性明显低于心梗后3d组[分别为(7.13±2.50),(14.12±3.55)μkat/g;(7.45±1.42),(16.59±7.17)μkat/g],但仍明显高于假手术组,差异有显著性意义(t=2.732,2.176,P<0.01);室间隔及右室中乙酰胆碱酯酶活性仍明显高于假手术组(P<0.01,P<0.05)。③心梗后30d时,梗死中心区和梗死周边乙酰胆碱酯酶活性较心梗后3d组进一步降低[分别为(4.55±1.17),(7.13±2.50)μkat/g;(5.83±1.55),(7.45±1.42)μkat/g],差异有显著性意义(t=2.653,2.763,P<0.05),梗死周边、室间隔、右心室中乙酰胆碱酯酶活性仍高于假手术组(P<0.05)。结论:心肌梗死后不同部位心肌中乙酰胆碱酯酶的活性具有不同的变化特点,提示心肌中乙酰胆碱酯酶的检测不仅是评价迷走神经活性的指标,可能也是一个较好的评价迷走神经支配的方法。

AIM： To observe the activity of acetylcholinesterase （AchE） in myocardium after acute myocardial infarction in rats. METHODS： The experiment was conducted in Central Laboratory of First Affiliated Hospital of Harbin Medical University from October 2004 to April 2005. A total of 68 male Wistar rats were used for the experiments. Myocardial infarction model was prepared by ligation of the left anterior descending coronary artery and the sham operation rat was only braided without ligation. According to the method of random digits table, 53 survival rats with myocardial infarction were divided into 3-day group, 7-day group and 30-day group with 9, 10 and 10 rats in each group, and 24 sham-operation rats were applied as control group with 8 in each group. The activities of AChE were examined in myocardum at infarcted zone, peri-infarcted area, interventricular septum and right ventricle. RESULTS： Totally 53 rats were involved in the result analysis. （1）In 3-day group, the activities of AChE in infarcted zone, peri-infarcted area and interventricular septum were significantly higher than those in the sham operation group [（14.12± 3.55）, （1.50±0.75） μkat/g; （16.59±7.17）, （0.92±0.22） μkat/g; （8.64±4.02）, （2.43±1.63） μkat/g] （t =3.168,3.367,P 〈 0.01,t =2.817,P 〈 0.05）. （2）In 7-day group, the activities of AChE in infarcted zone and peri-infarcted area were significantly lower than those in 3-day group [（7.13±2.50）, （14.12±3.55） μkat/g; （7.45±1.42）, （16.59±7.17） μkat/g], but obviously higher than those in the sham operation group （t =2.732,2.176,P 〈 0.01 ）. The activities of AChE in septum and right ventricle were significantly higher than those in the sham operation group （P 〈 0.01,P 〈 0.05）. （3）In 30-day group, the activities of AChE in infarcted zone and peri-infarcted area were significantly lower than those in 3-day group [（4.55_＋1.17）,（7.13±2.50） μkat/g;（5.83±1.55）,（7.45±1.42） μkat/g] （t =2.653,2.763,P〈 0.05）. The activities of Ache in peri-infarcted area, septum and right ventricle were still higher than those in the sham operation group （P 〈 0.05）. CONCLUSION： The activities of AChE in different zones of myocardium are distinct after myocardial infarction, which indicates that the activity of AChE may not only be a value of vagal activity, but also be a method to evaluate vagal innervation.