鸭乙肝模型氧化应激反应及金丝桃苷的干预

Interventional effect of hyperin on oxidative stress in duck hepatitis B virus models

目的:观察鸭乙肝病毒感染所致稚鸭血清及肝脏氧化应激状态的改变及金丝桃苷的干预作用。方法:实验于2006-03/07在中国医学科学院生物技术研究所以及解放军第三○一医院老年医学研究所进行。①实验分组:44只1日龄北京稚鸭随机分为7组:正常组,模型组,金丝桃苷15,30,60mg/(kg·d)剂量组,拉米夫定50mg/(kg·d)组和恩替卡韦0.25mg/(kg·d)组。其中正常组5只,金丝桃苷15,30,60mg/(kg·d)剂量组各7只,其余各组6只。②实验干预:除正常组外,其余6组每只动物经胫静脉注射鸭乙肝病毒阳性血清0.2mL,第7天开始灌胃给药,2次/d,给药10d,正常组和模型组给予等量生理盐水。停药后第3天处死动物检测相关指标。③实验评估:黄嘌呤氧化酶法测定超氧化物歧化酶活性、非酶促反应法检测谷胱甘肽过氧化物酶活性,硫代巴比妥酸法检测丙二醛含量。结果:纳入稚鸭44只,在模型制备及后期干预中无动物死亡,全部纳入统计分析。与正常组相比,模型组肝匀浆超氧化物岐化酶活性、谷胱甘肽过氧化物酶活性及丙二醛含量显著降低(P<0.01,P<0.05),而血清谷胱甘肽过氧化物酶活力及丙二醛含量显著升高(P<0.01,P<0.05);与模型组相比,金丝桃苷治疗组肝匀浆超氧化物岐化酶、谷胱甘肽过氧化物酶活性及丙二醛含量显著升高(P<0.01,P<0.05),然而血清谷胱甘肽过氧化物酶活性及丙二醛含量显著降低(P<0.01,P<0.05)。结论:鸭乙肝病毒感染引起稚鸭肝脏氧化应激损伤,金丝桃苷可有效的逆转肝损伤。

AIM： To observe the changes of oxidative stress in serum and liver homogenate of ducklings due to duck hepatitis B virus （DHBV） infection, and the intervention of hyperin （Hyp）. METHODS： The experiment was performed at the Biotechnology Institute of Chinese Academy of Medical Science and Institute of Geriatrics of the 301 Hospital of Chinese PLA from March to July 2006. （1） Totally 44 1-day Beijing ducklings were divided into 7 groups randomly： normal group （n =5）, model group, Hyp low-dose group （15 mg/kg per day） （n =7）, Hyp medium-dose group （30 mg/kg per day） （n =7）, Hyp high-dose group （60 mg/kg per day） （n = 7）, lamivudine group （50 mg/kg per day） （n =6） and entecavir group （0.25 mg/kg per day） （n =6）. （2） All but ducklings in normal group were infected with DHBV positive serum 0.2 mL. Intragastric administration started 7 days later, b.i.d, for 10 days successively. Animals in normal group and model group were administrated with the same amount of saline. Animals were sacrificed 3 days after the last administration, and then relevant indexes were determined. （3） Activities of superoxide dismutase （SOD） and glutathion peroxidase （GSH-PX）, content of malondialdehyde （MDA） were assayed by xanthine oxidase method, non-enzymatic reaction method and thio-barbituric acid method, respectively. RESULTS： A total of 44 ducklings were involved in the result analysis, no drop out. （1）Compared with normal group, activities of SOD and GSH-PX, content of MDA in liver homogenate of model group were significantly decreased （P 〈 0.01, P 〈 0.05）, while serum activity of GSH-PX and content of MDA were significantly increased （P 〈 0.01, P 〈 0.05）. Compared with model group, activities of SOD and GSH-PX, and content MDA in liver homogenate were increased significantly after Hyp treatment （P 〈 0.01, P 〈 0.05）, while activity of GSH-PX and content of MDA in serum were significantly decreased （P 〈 0.01, P 〈 0.05）. CONCLUSION： DHBV infection leads to hepatocyte oxidative stress, while Hyp is effective in combating hepatic injury.