江苏地区汉族人群白细胞分化抗原14启动子C(-159)T多态性与冠状动脉粥样硬化性心脏病的相关性

Association of C(-159)T polymorphism in the promoter of CD14 gene with coronary heart disease in Han population of Jiangsu province

目的:分析江苏地区汉族人群白细胞分化抗原14基因启动子C(-159)T多态性与冠状动脉粥样硬化性心脏病(冠心病)之间的关系。方法:选择2003-08/2005-09南京市鼓楼医院心内科经冠状动脉造影确诊为冠心病患者(冠状动脉至少有一支管腔狭窄大于50%)193例。同时间随机抽取参加南京市鼓楼医院门诊无血缘关系的健康体检者225名为健康对照组。采用聚合酶链反应-限制性片段长度多态性技术分析冠心病患者及健康对照者白细胞分化抗原14基因启动子-159位点多态性。并采用生化方法和酶联免疫吸附法检测研究对象的血脂和外周血可溶性白细胞分化抗原14水平。结果:所有受试者完成测试并进入结果分析,无脱落者,DNA抽提均成功。①血脂和可溶性白细胞分化抗原14水平:冠心病组血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、血清白细胞分化抗原14水平高于健康对照组。血清高密度脂蛋白胆固醇水平低于健康对照组。②基因型分布:健康对照组和冠心病组CC,CT,TT基因型分布分别为20.90%,55.10%,24.00%和15.00%,49.20%,35.80%,健康对照组高于冠心病组,差异有统计学意义(χ2=7.527,P<0.05)。③等位基因频率:健康对照组和冠心病组C,T等位基因频率则分别为48.40%,51.60%和39.40%,60.60%,健康对照组高于冠心病组,差异有统计学意义(χ2=6.858,P<0.05)。④冠心病组CC,TC和TT基因型人群之间血脂水平和血清可溶性白细胞分化抗原14水平差异无显著性(P均>0.05)。结论:白细胞分化抗原14基因启动子C(-159)T位点多态性与江苏地区汉族人群冠心病存在相关性,它可能是汉族人群冠心病发病的遗传学易感因素。

AIM： To investigate the association between the C （-159）T polymorphism in the promoter of CD14 gene and coronary heart disease （CHD） in the Han population of Jiangsu province. METHODS： Between August 2003 and September 2005, 193 CHD patients were recruited from Nanjing Drumtower Hospital. They all had 〉 50% stenosis in at least one major coronary artery and were diagnosed by coronary angiography. Meanwhile 225 uncorrelated subjects who received routine health examination in the same hospital were taken as healthy control group. Using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method, the C（-159）T polymorphism in the promoter of CD14 gene were analyzed in CHD and control groups, the levels of serum lipid and soluble CD14 were also measured by biochemical and enzyme linked immunosorbent assay methods respectively. RESULTS： All the subjects completed the test and entered the result analysis. （1）The levels of serum lipids and soluble CD14： Compared with control group, CHD group had higher levels of serum total cholesterol, triglyceride, low-density lipoprotein cholesterol, and CD14, but the level of high-density lipoprotein cholesterol was lower in CHD group.（2） Genotype analysis： 3 genotypes were observed in the study： CC genotype, CT genotype, and TT genotype were 20.90%, 55.10%, 24.00% and 15.00%, 49.20%, 35.80% in control group and CHD group, respectively. And the control group was significantly higher than CHD group （X^2 =7.527, P 〈 0.05）.（3）The genotype frequencies of C and T alleles were 48.40%, 51.60% and 39.40%, 60.60% in control group and CHD group, respectively. The difference between the two had a statistical significance （X^2 =6.858, P 〈 0.05）.（4）No significant differences for the levels of serum lipids and soluble CD14 were found among TT, TC, and CC genotypes in CHD patients （P 〉 0.05）. CONCLUSION： The C （-159）T polymorphism in the promoter of CD14 gene is associated with CHD in the Han population of Jiangsu province, and it might be a genetic predisposing predictor for CHD in the Han population.