益精养血方对帕金森病大鼠多巴胺能神经元凋亡的影响

Effects of Yijingyangxue prescription on apoptosis of the dopaminergic neurons in rats with Parkinson disease

目的:研究益精养血方对帕金森病(Parkinson disease,PD)大鼠多巴胺能神经元凋亡的影响。方法:采用6-羟多巴胺制备的大鼠PD模型,实验分为模型组、实验(中药)组和正常组。正常组和模型组大鼠生理盐水灌胃,实验组大鼠益精养血方灌胃,各30 d。灌胃结束2周后行行为学检测,免疫组织化学方法观测黑质酪氨酸羟化酶(TH)、Bcl- 2、Caspase-3的表达,免疫电镜技术观察黑质多巴胺能神经元结构变化。结果:实验组大鼠的旋转圈数比治疗前明显减少;实验组损毁侧与健侧黑质TH阳性细胞数量较模型组显著提高;实验组损毁侧黑质Caspase-3表达的D值比模型组显著降低,而Bcl-2的表达与Caspase-3相反;免疫电镜观察TH免疫反应产物表达于黑质DA能神经元高尔基复合体质膜面及胞质内,电子密度较高,在正常组中多见,实验组较少,而模型组很少见。结论:益精养血方对PD大鼠多巴胺能神经元凋亡有明显抑制作用。

Objective： To explore the effects of Yijingyangxue prescription on expression of Bcl-2 and Caspase-3 of the dopaminergie neurons in rats with Parkinson＇s disease （PD）. Methods： PD models were established by mieroinjeetion of 6-hydroxydopamine （6-OHDA） into the substantia nigra of the right side of the brain. The model group, experimental （Yijingyangxue prescription） group and the normal group were set up. The model and normal group rats were given normal saline and the experimental group rats were given Yijingyangxue prescription by gastric gavage for 30 days. Rotational behavior of rats was examined. The expressions of TH, Bcl-2 and Caspase-3 in the substantia nigra were detected using immuohistochemical method. Ultrastructural changes of dopaminergic neurons in the pars compacta of the substantia nigra （SINe） were observed. Results： The experimental group rats showed less rotational behavior after therapy （P〈0. 05）. The survival percentage （6-OHDA injected side in comparison with normal side） of the number of TH immunoreactive cells in the SINe of experimental group rats was higher than that of the model group （P〈0. 05）. The OD value of Caspase-3 in the SNc of 6 OHDA injected sides obviously decreased in the experimental group compared with the model group （P〈0. 05）. The changes of Bcl-2 were opposite to those of Caspase-3. Under immunoeleetronic microscopy, TH immunoreactive products were located on the membrane of Golgi apparatus and cytoplasm of TH positive cells, and their electron density was condensed. TH immunoreactive products were found in the normal group, less in the experimental group, but lest in the model group. And the ultrapathological changes in the experimental group were less than those of the model group. Conclusion： Yijingyangxue prescription could obviously inhibit the apoptosis of dopaminergic neurons in the SNc of PD rats.