低能N^+注入诱变选育威兰胶高产菌的研究

Breeding of welan gum-producing bacteria by low energy N^+ ion implantation

利用低能N^+束对威兰胶生产菌进行了辐射诱变选育研究。能量为20keV,注入剂量为20x10^(14)/cm^(-2)时,筛选出一株高产菌株Alcaligenes sp.NX-3-1。结果表明,摇瓶发酵中诱变菌威兰胶产量比出发菌高27.5%;在7.5L发酵罐上,Alcaligenes sp.NX-3-1菌生长速度比出发菌快,生物量高,底物葡萄糖消耗量多,威兰胶产量较出发菌有明显提高,最高达26.4g·L^(-1)。

Breeding of welan gum-producing bacteria by low energy N^＋ ion implantation has been investigated. A high production mutant NX-3-1 for welan gum was screened under the implantation conditions of 20keV, 20×10^14 /cm^-2, the average production of welan gum with NX-3-1 has been raised by 27.5% compared to the original strain in shaking flask culture. NX-3-1 grew much faster than original strain companied with higher glucose consumption in 7.5L fermenter. The maximum welan gum concentration with NX-3-1 was 26.4 g . L^-1.