900MHz微波辐射对鸡胚脑组织氧化应激及出壳后小鸡自发活动的影响

The effect of exposure with 900MHz microwave on oxidative stress of the brain in chick embryos and spontaneous activity in chick

研究900MHz微波辐射对鸡胚脑组织超氧化物岐化酶(Superoxide dismutase,SOD)、鸟氨酸脱羧酶(Ornithine decarboxylase,ODC)活性以及出壳后小鸡自发活动的影响。采用海兰白鸡受精卵30只,随机分为6组。其中对照组和照射组各3组,每组5只。各处理组鸡胚从孵化5-20d,每天接受2h整体微波辐射。在孵化第13d、20d采集脑组织;黄嘌呤氧化酶法测定SOD活性,NGO分光光度法测定ODC活性。小鸡出壳后2d,自发活动测量系统测量小鸡自发活动度。结果发现,在孵化第13d,照射组鸡胚脑组织SOD活性与对照组相比显著降低,20d恢复正常。照射组鸡胚出壳后小鸡的自发活动度,与对照组小鸡相比显著增强。以上结果提示,900MHz微波辐射可以引起13d鸡胚脑组织氧化应激反应,增强出壳后小鸡的自发活动。

The study of biological effects induced by microwave （900MHz） on SOD （Superoxide dimutase） and ODC （Ornithine decarboxylase） activity in chick embryos＇ brain, and spontaneous activity of the newborn chicken has been reported in this paper. Thirty chick embryos were randomly divided into 6 groups, 3 control groups and 3 exposure groups. 3 exposure groups were irradiated with 900MHz microwave 2h per day from 5 to 20 day. The SOD and ODC activity in chick embryos＇ brain was examined at 13^th and 20^th day of the incubation and at the second day after birth spontaneous activity was monitored. The SOD activity of the exposed groups significantly decreased at the 13th day and was recovered at the 20^th day. The spontaneous activity in chicken of the exposure group increased significantly compared to the control group. Microwave would induce oxidative stress in chick embryos and enhance spontaneous activity of the chicken hatched.