血清中HCV抗体与HCV-RNA检测在临床中的应用

Clinical Application of Blood Serum Anti-HCV and HCV-RNA Examination in Diagnosis of Patients With HCV

目的探讨丙型肝炎病毒抗体与RNA在临床中的应用价值。方法用ELISA法检测934例丙型肝炎可疑患者病毒抗体,用实时荧光定量PCR检测379例丙型肝炎可疑患者病毒RNA。结果934例患者HCV抗体阳性率为19.16%,379例HCV-RNA阳性率为43.01%。53例为两者均做的病人,RNA和Ab均阳性的占52.8%(28/53),均阴性占15.1%(8/53),两者的一致率达67.9%,Ab的阳性率为81.1%(43/53),RNA的阳性率为56.6%(30/53)。结论HCV抗体联合HCV-RNA检测对丙型肝炎的临床诊断有一定的价值。

Objective To investigate the clinical value of serum anti- HCV and HCV - RNA in the diagnosis of hepatitis C patients. Methods Hepatitis C antibody was detected in 934 suspicious patients by ELISA, and HCV- RNA was detected in 379 suspicious patients by real time FQ- PCR. Results The positive rates of HCV antibody and HCV RNA were 19. l 6 % （179/934） and 43.01% （163/379） respectively. Fifty - three patients were tested HCV antibody and HCV - RNA; the positive rate of both of them was 52.8% （28/53）, and the negative rate of both of them was 15. 1% （8/53）. The consistency rate was 67.9%, the positive rates of HCV antibody and HCV - RNA were 81. 1% （43/53） and 56.6% （30/53） respectively. Conclusions Detection of antibodies against HCV and HCV- RNA plays an important role in clinical diagnosis of hepatitis C.