β-catenin和γ-catenin基因在鼻咽癌及其淋巴结转移中的表达及意义

The expression of β-catenin and γ-catenin gene in nasopharyngeal carcinoma and lymph node with NPC metastasis and their biological significance

目的探讨β-连接素（β-catenin）和γ-连接素（γ-catenin）基因在鼻咽癌及其淋巴结转移中的表达及意义。方法应用免疫组化SP法检测15例鼻咽黏膜慢性炎、28例分化型非角化性鼻咽癌和20例鼻咽癌淋巴结转移中β-catenin和γ-catenin的表达。结果β-catenin、γ-catenin在分化型非角化性鼻咽癌及其淋巴结转移中的异常表达率均分别为85.7%（24/28）和95.0%（19/20）,均显著高于鼻咽黏膜慢性炎[分别为13.3%（2/15）和6.7%（1/15）]（P〈0.01）。β-catenin、γ-catenin在鼻咽黏膜慢性炎中的染色分级分别为2.80±0.56和2.93±0.26;分化型非角化性鼻咽癌为0.79±1.07和0.86±1.15;鼻咽癌淋巴结转移为0.25±0.72和0.30±0.73;三组别中两两比较差异有统计学意义（P〈0.05）。β-catenin和γ-catenin在鼻咽黏膜慢性炎、分化型非角化性鼻咽癌及其淋巴结转移中均呈正相关关系（rs=0.89,0.61,0.94,P〈0.01）。结论β-catenin、γ-catenin基因异常表达可能共同参与鼻咽癌的侵袭和转移;β-catenin和γ-catenin染色分级可能为判断鼻咽癌转移的更敏感指标。

Objective To investigate the expressions of β-catenin and γ-catenin gene in differentiated non - keratinizing nasopharyngeal carcinoma （NPC） and lymph nodes with NPC metastasis, and their biological significance. Methods Expressions of β-catenin and γ-catenin were detected by immunohistochemical method in 15 patients with chronic nasopharyngitis, 28 patients with differentiated non - keratinizing carcinoma and 20 patients with lymphy node metastasis of the NPC. Results In differentiated non - keratinizing carcinoma and lymph node metastasis of the NPC, the abnormal expression rates of β-catenin and γ-catenin were 85.7% （ 24/28 ） and 95.0% （19/20） respectively, which were significantly higher than that of chronic nasopharyngitis（ 13.3% ,2/15 and 6.7%, 1/15）respectively）（ P 〈0. 01 ）. In differentiated non - keratinizing carcinoma and lymph node metastasis of the NPC, the staining grades of β-catenin and γ-catenin were 2. 80 0. 56 and 2. 93 ± 0. 26 in chronic nasopharyngitis, 0. 79 ± 1.07 and 0. 86 ± 1.15 in differentiated non-keratinizing carcinoma and 0. 25 ± 0. 72 and 0. 30 ± 0. 73 in lymph node metastasis of the NPC, respectively. The staining grades of β-catenin and β-catenin among the three groups had significant differences（ P 〈0. 01 or P 〈0. 05 ）. The expression of β-catenin and β-catenin showed a positive correlation（ P 〈 0. 01 ） in chronic nasopharyngitis, differentiated non - keratinizing carcinoma and lymph node metastasis of the NPC. Conclusions The abnormal expression of [^-catenin and β-catenin may together participate in the invasion and metastasis of NPC. The staining grades of β- catenin and β-catenin may be more sensitive at predicting metastasis of NPC.