葛根黄酮预处理对大鼠肾脏缺血再灌注损伤的保护作用

Protective Effects of Pueraria Flavonoid on the Renal Ischemia Reperfusion Injury Model in Rats

目的:探讨葛根黄酮预处理对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法:30只SD大鼠随机分为3组(n=10):假手术组、缺血再灌注损伤(IR)组、葛根黄酮预处理(PFI)组。建立大鼠肾脏缺血再灌注损伤模型,测定血清肌酐(Scr)与尿素氮(BUN)含量、肾组织匀浆中SOD、GSH-Px的活性及MDA的含量。结果:与IR组比较,PFI组Scr(P<0.01)、BUN(P<0.05)、MDA(P<0.05)含量显著下降。而SOD(P<0.01)、GSH-Px(P<0.05)活力显著升高。结论:SOD、GSH-Px是重要的内源性保护物质,葛根黄酮可增加SOD、GSH-Px的活性、增加机体抗氧化和清除自由基的能力,从而发挥对肾缺血再灌注损伤的预防和保护作用。

Objective：to investigate the protective effects and its mechanism of pueraria flavonoid on renal ischemia reperfusion injury in rats.Method：30 SD rats were divided into three groups randomly：sham operation group,ischemia reperfusion injury group（IR）,and pueraria flavonoid injection preconditioning group（PFI）.The model of renal ischemia reperfusion injury was produced.The serum Scr and BUN levels were measured,the renal superoxide dismutase（SOD）,GSH-Px activities and malondial dehyde（MDA） content were detected respectively.Result：The contents of Scr（P〈0.01）,BUN（P〈0.05）,MDA（P〈0.05） were significantly lower,the activities of SOD（P〈0.01）and GSH-Px（P〈0.05） were significantly higher in PFI group than that in IR group.Conclusion：SOD and GSH-Px are important substabces of endogenous peotection,and pueraria flavonoid can enchance SOD and GSH-Px activities,and protect renal ischemia reperfusion injury.