石油污染土壤中喹啉降解菌Q5的筛选及其喹啉降解性能

SCREENING OF STRAIN Q5 AND ITS DEGRADATION ABILITY FOR QUINOLINE FROM OIL CONTAMINATED SOIL

喹啉是重要的化工原料,但其也带来严重的环境污染。采用富集培养的方法,从胜利油田石油污染土壤中筛选分离到1株喹啉高效降解菌Q5,初步鉴定为革兰氏阳性芽孢杆菌。在好氧条件下,对菌株Q5进行喹啉生物降解实验,以确定最佳降解条件。结果表明,在30℃、碱性环境、接种量10%(体积分数)、转速160r/min和适当底物浓度下,菌株Q5有较高的喹啉降解率;不同的碳氮源对菌株Q5的喹啉降解效果有不同的影响,添加无机氮源有助于提高Q5的喹啉降解率。在生物修复被喹啉及其衍生物污染的水体及土壤的过程中,菌株Q5具有较高的潜在应用价值。

Quinoline is an important raw material in chemical industry, which can also cause severe pollution of environment. The strain Q5 with high biodegradation efficiency for quinoline was screened from a lot of contaminated soil samples in Shengli oilfield. According to the form, the physiological and biochemical characteristics of Q5, it was identified as Bacillus. The culture condition for strain Q5 and the conditions for quinoline degradation were investigated under aerobic atmosphere. The results indicated that the optimal degradation conditions were as follows： 30℃, alkaline medium, 10% inoculum amount, 160 r/min and suitable concentrations of substrates in culture solution. The quinoline degradation of strain Q5 was related to the carbon and nitrogen sources in culture solution. Adding inorganic nitrogen source （NH4）2SO4 could enhance the quinoline degradation rate of QS. During the bioremediation of the water system and soil contaminated by quinoline or its derivates, Q5 is expected to play an important role.