血管内皮生长因子C和nm23表达与微血管密度及宫颈癌转移的关系

Expression of VEGF-C,nm23 gene and microvessel density in cervix carcinoma and their relation with metastasis

目的探讨血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)和nm23(non-metastasis23)表达与微血管密度(microvessel density,MVD)及宫颈癌转移的关系。方法采用免疫组化SABC法检测16例正常宫颈上皮及68例宫颈癌中VEGF-C、nm23的表达。并用CD105标记新生血管内皮细胞,计算肿瘤内MVD。结果VEGF-C在宫颈癌组织中的阳性表达率显著高于正常组织(P<0.01),淋巴结转移组VEGF-C阳性率高于无淋巴结转移组(P<0.05),VEGF-C表达强度与MVD呈正相关(P<0.05)。nm23蛋白表达率显著低于正常组织(P<0.01),淋巴结转移组nm23阳性率低于无淋巴结转移组(P<0.05)。nm23在宫颈癌中的表达与VEGF-C呈负相关(P<0.01)。结论宫颈癌组织VEGF-C呈高表达、nm23呈低表达,可能在宫颈癌血管生长、肿瘤浸润转移过程中起重要作用。检测宫颈癌中VEGF-C、nm23表达对进一步了解宫颈癌局部血管生成情况及判断预后有一定的应用价值。

Objective To investigate expressions of vascular endothelial growth factor-C （VEGF-C） non-metastasis （nm23） gene and microvessel density （MVD） in cervix carcinoma, simultaneously, their relation with metastasis was also studied. Methods the expression of VEGF-C, nm23 in 68 cases of cervical carcinomas and 16 cases of normal cervical epithelium were detected by immunohistochemistry SABC method, and blood vessel endothelial cells were labeled by CDI05 and MVD of the cervix carcinoma was assayed. Results The positive expression rates of VEGF-C was remarkably higher in cervical carcinoma than those in normal cervical epithelium （P〈0.01）, the positive rates of VEGF-C in cases with lymph node metastasis was higher than that in the cases without lymph node metastasis （P〈0.05）, there was positive correlation between the expression intensity of VEGF and MVD. While the positive expression rates of nm23 was remarkably lower in cervical carcinoma than those in normal cervical epithelium （P〈0.01）, the positive rates of nm23 in cases with lymph node metastasis was lower than that in the cases without lymph node metastasis （P〈0.05）, there was a negative correlation between nm23 expression and VEGF-C expression （P〈0.05）. Conclusion The higher expression of VEGF-C and the lower expression of nm23 in cervical carcinoma may play an important role during tumor angiogenesis, invasion and metastasis. Detection for expression of VEGF-C nm23 may be valuable for further understand the regional tumor angiogenesis and predict the prognosis of cervix carcinoma.