癌基因C-myc在结直肠癌组织中的表达及临床意义

The clinical significance of C-myc protein expression in colorectal carcinoma

目的探讨结直肠癌组织中癌基因C-myc在结直肠癌发生、发展中的作用及其与临床病理特征的相关性。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶法(S-P法),检测60例结直肠癌组织、10例癌旁组织及8例正常结直肠黏膜中C-myc蛋白表达。结果结肠癌组织中,C-myc蛋白表达阳性率为76.7%(46/60),显著高于正常及邻近结直肠组织(P<0.01);C-myc的过度表达与结直肠癌肝转移呈正相关关系(r=5.540,P<0.05),与TNM分期无相关性(r=0.013,P>0.05)。结论癌基因C-myc在结直肠癌的发生、发展过程中起重要作用,C-myc蛋白过度表达不能代表结直肠癌的肿瘤状态,不能作为判断病人预后的单独指标。

Objective To investigate the correlation between the expression of oncogene C-myc and clinicaopathological features in colorectal carcinoma. Methods The expression C-myc protein in normal colorectal mucosa（ n = 8）, the adjacent non-cancerous tissues（ n = 10） and the primary colorectaX carcinoma tissues（ n = 60） was observed by S-P inanunohistochemical assay.Results Of 60 colorectal carcinoma tissues C-myc protein was detected in 46 cases（76.7% ）. The expression rate of C-myc in the primary col- orectal carcinoma tissues was significantly higher than that in the adjacent non-cancerous tissues（ P 〈 0.01 ）. Overexpression of C-myc was significantly correlated with hepatic metastasis（ r = 5. 540, P 〈 0.05） and was not related with TNM staging（ r = 0. 013, P 〉 0.05）. Conclusion Oncogene C-myc play an important role in the carcinogenesis and development of colorectal carcinoma. There was no correlation for C-myc protein expression with the clinicopathological parameters of patients. The expression of C-myc could not be used as an exclusive marker of tumour burden of colorectal carcinoma.