槐定碱致痫大鼠海马Glu、GABA及血清中TNF-α、IL-2和IL-6含量变化的研究

Study on Contents of Glu and GABA of Hippocampus and Serum IL-2,IL-6 and TNF-α Level in Epileptic Rats Induced by SRI

目的探讨槐定碱(Sophojridine,SRI)致痫大鼠海马组织中谷氨酸(Glu)和γ-氨基丁酸(GABA),血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和白细胞介素-6(IL-6)的变化。方法腹腔注射槐定碱(47.83mg/kg)诱导大鼠癫痫,采用放射免疫测定法检测TNF-α、IL-2和IL-6表达的水平,免疫组化法测定Glu和GABA阳性神经元的含量。结果模型组大鼠血清中TNF-α、IL-2和IL-6含量均明显高于对照组(P<0.01),大鼠海马组织中Glu免疫反应阳性细胞数,模型组均明显高于对照组,GABA免疫反应阳性细胞数,模型组均明显低于对照组(P<0.05)。结论TNF-α、IL-2、IL-6可能参与了癫痫的发病;Glu和GABA等氨基酸类神经递质平衡紊乱可能是癫痫发病的基础之一。

Objective To investigate the changes of the contents of glutamate（Glu）、 gama-aminobutyric（GABA）,serum IL-2,IL-6 and TNF-α in epileptic rats induced by sophoridien（SRI）.Methods We establish epileptic rats model induced by SRI with intraperitoneal injection.The levels of serum TNF-α,IL-2 and IL-6 of each group were assayed by radioimmuoassay（RIA）.Meanwhile we measured the contents of glutamate（Glu） and gama-aminobutyric（GABA） of the hippocampus by immunohistochemistry.Results The levels of serum TNF-α,IL-2 and IL-6 were significantly higher in model group than those in control group（P〈0.01）.Glu positive cells in hippocampus in model group were more than those in control group.Furthermore,GABA positive cells in model group were less than those in control group（P〈0.05）.Conclusion TNF-α,IL-2 and IL-6 may be involved in the pathogenesis of epilepsy.Neurotransmitter balance disorderin of the Glu and GABA may be the base of pathogenesis of epilepsy.