稀硫酸水解稻壳制备可发酵性糖

Pretreatment of Rice Hull by Dilute Sulfuric Acid for the Production of Fermentable Sugar

考察了稻壳在稀H2SO4条件下高温水解的反应过程,通过单因素实验和正交实验确定较优的酸解条件为:H2SO4用量1.0%、反应时间60 min、稻壳添加量15%、温度140℃,并对该条件进行了验证。采用纤维素酶对稻壳的酸解产物进行糖化,进一步提高了可发酵性糖的产量,最终糖的总得率可达67.06%。

The hydrolysis of rice hull by dilute H2SO4 under high temperature was investigated in detail. Through the single factor experiments and the L9 （3^4 ） orthogonal experiments, the optimal hydrolysis conditions for rice hull by dilute H2SO4 were achieved as follows： H2SO4 amount 1.0% ,reaction time 60 min,amount of rice hull 15% and temperature 140℃. The combination of above conditions was subsequently verified to Obtain higher yield of sugars. Then,the pretreated rice hull was further saccharified with cellulase to gain more fermentable sugar. As a whole,the total yield of fermentable sugar from rice hull was 67.06% ,according to the crude fiber in rice hull.