辛伐他汀对成骨细胞增殖和矿化功能影响的实验研究

Simvastatin promotes the proliferation and mineralization of osteoblasts cultured in vitro

目的:研究辛伐他汀对原代培养大鼠颅骨成骨细胞增殖和矿化功能的影响。方法:酶消化法分离培养大鼠颅骨成骨细胞,分别用空白培养基、不同浓度辛伐他汀及不同浓度的rhBMP-2作用24 h后,MTT法测量药物对细胞增殖活性的影响,细胞接种到培养板连续培养7 d,von Kossa染色观察成骨细胞矿化结节,采集图像并转换处理后计算矿化结节面积的百分比。结果:辛伐他汀对成骨细胞增殖活力的增加呈剂量依赖方式,而且可使成骨细胞矿化面积百分比显著增加,与rhBMP-2的作用相当。结论:辛伐他汀可促进体外培养大鼠颅骨成骨细胞的增殖和矿化,从而发挥促进骨形成的作用。

Objective： To study the effect of Simvastatin on the proliferation and mineralization of cultured rat calvaria osteoblasts （RCOBs）. Methods： RCOBs were cultured in vitro by an enzyme-digestion method and treated with Simvastatin and rhBMP-2 in series gradient of concentration for 24 hours. Then the viability and proliferation of RCOBs were determined by MTT assay. After implantation in a 12-well microtiter plate for 7 days, the mineralization nodes were stained by the von Kossa method. Photos were transferred into a black-white style and the mean mineralization area ratio was measured. Results： Simvastatin could not only stimulate the proliferation of the RCOBs in a dose-dependent manner, but also increased the mineralization ratio compared with the control group, which mimicked rhBMP-2. Conclusion： Simvastatin can promote the proliferation and mineralization of RCOBs in vitro, which may partially be attributed to the osteogenesis mechanism of statins.