摘要
目的探讨结直肠细胞癌周期蛋白E、生存素及PTEN基因蛋白表达与临床病理特征及预后的相关性。方法采用链霉菌抗生物素蛋白-过氧化酶法(S-P法)检测周期蛋白E、生存素及PTEN基因蛋白在有完整随访资料的69例结直肠癌组织中的表达,并作单因素和多因素生存分析。结果周期蛋白E或PTEN表达与结直肠癌患者年龄、组织学分级及TNM分期有关(P<0.05),生存素表达与组织学分级及TNM分期有关(P<0.05)。周期蛋白E或生存素表达与PTEN表达之间呈显著负相关(P<0.05)。单因素分析显示,患者生存率与年龄、组织学分级、浸润深度、TNM分期及生存素或PTEN的表达均有关(P<0.05)。但经多因素Cox比例风险模型分析,只有组织学分级、TNM分期及PTEN的表达具有独立的预后意义。结论周期蛋白E、生存素及PTEN基因蛋白在结直肠癌中的表达与一些临床病理特征有关。组织学分级为高级别组、TNM分期为Ⅲ、Ⅳ期及PTEN失表达可作为结直肠癌预后不良的独立性指标。
To investigate the relationship between the expression of cyclin E,survivin and PTEN gene proteins and the clinicopathological features and prognosis in patients with colorectal carcinoma.Methods The expressions of cyclin E,survivin and PTEN gene proteins were detected using the standard streptavidin peroxidase（SP）method in 69 cases of primary colorectal carcinoma.All cases were followed up for more than 10 years,in which the prognosis was analyzed with univariate and multivariate analysis.Results Expression of cyclin E or PTEN was correlated with age,histological grading and TNM staging（P〈0.05）.Expression of survivin was correlated with histological grading and TNM staging（P〈0.05）.There was a negative correlation between the expression of cyclin E or survivin and the expression of PTEN（P〈0.05）.Univariate analysis indicated that the survival rate was correlated with age,histological grading,infiltration depth,TNM staging and expression of survivin or PTEN.On Cox multivariate analysis,histological grading,TNM staging and expression of PTEN were independent prognostic factors.Conclusion The expression of cyclin E,survivin and PTEN in colorectal carcinoma is significantly correlated with some clinic opathological characteristics.High histological grading,TNM Ⅲ or Ⅳ stage and loss expression of PTEN may be taken as the independent parameters of poor prognosis in patients with colorectal cancer.
出处
《江苏医药》
CAS
CSCD
北大核心
2007年第10期998-1001,F0003,共5页
Jiangsu Medical Journal
基金
南通市社会发展科技计划(S40024)
