人单核细胞白血病细胞系SHI-1的多药耐药和耐药蛋白的表达

Preliminary study on multidrug resistance in a novel human acute monocytic leukemic cell line SHI-1

目的探讨人单核细胞白血病细胞系SHI-1多药耐药谱及其机制。方法噻唑蓝(MTT)检测SHI-1细胞对柔红霉素、鬼臼乙叉甙、高三尖杉酯碱、紫杉醇和长春新碱的敏感性;流式细胞术(FCM)检测SHI-1细胞中P糖蛋白(P-gp)、多药耐药相关蛋白(MRP)、肺耐药相关蛋白(LRP)、乳腺癌耐药蛋白(BCRP)、谷胱甘肽-S-转移酶-π(GST-π)等耐药蛋白的表达。结果MTT药敏实验结果显示SHI-1细胞存在着多药耐药,尤其对VP-16最为明显。FCM检测显示SHI-1细胞中P-gp阳性比例10.78%,相对荧光强度(RFI)为1.02;GST-π阳性率93.52%,RFI6.91;LRP阳性率92.86%,RFI8.00;MRP阳性率4.54%,RFI1.38;BCRP阳性率3.84%,RFI1.06。结论SHI-1细胞存在多药耐药,以对VP-16最为明显,其耐药机制可能与LRP和GST-π的高表达有关。

To explore the multidrug resistance of a novel human monocytic leukemic cell line SHI-1 and its mechanisms.Methods The sensitivity of SHI-1 cell to various chemotherapeutic drugs was evaluated by thizolyl blue method（MTT）.FCM was used to study the expression of P-glycoprotein（P-gp）,multidrug resistance related protein（MRP）,lung resistance protein（LRP）,GST-π and breast cancer resistance protein（BCRP）.Results The results of MTT disclosed that there was a multidrug resistance in the SHI-1 cell line especially for etoposide.The results of FCM showed that the SHI-1 cell line was positive for LRP（92.86%,RFI 8.00）and GST-π（93.52%,RFI 6.91）,but negative for P-gp（10.78%,RFI 1.02）,MRP（4.54%,RFI 1.38）,and BCRP（3.84%,RFI 1.06）.Conclusion The multidrug resistance is existed in the SHI-1 cell line especially for etoposide.The mechanism of multidrug resistance may be associated with the upregulation of expression of the LRP gene and the GST-π gene.