三种脑缺血大鼠模型的血液流变学比较

Hemorheological Comparison of Three Kinds of Rat Model with Cerebral Ischemia

目的对三种脑缺血大鼠模型的血液流变学(全血黏度、血浆黏度、红细胞比容、红细胞变形指数)进行比较,筛选出血液流变学变化明显的符合临床特征的脑缺血模型。方法选择线栓法大脑中动脉阻塞(MCAO)大鼠模型、双侧颈总动脉(DCCA)结扎大鼠模型、单侧颈总动脉(SCCA)结扎大鼠模型三种脑缺血模型,进行脑缺血后1、3、5、7 d不同时间点血液流变学的比较。结果MCAO组、DCCA组、SCCA组与正常组大鼠的血液流变学比较均存在统计学差异(P<0.05);不同时间点血液流变学结果显示:MCAO组内全血黏度值指标第3 d为最高值(19.29±2.996)mPa.s/5s-1,且第3 d血浆黏度值(1.81±0.348)mPa.s、红细胞比容(47.5±5.28)%为模型组间最高值;MCAO组大鼠红细胞变形指数(0.47±0.017)较正常组及SCCA组、DCCA组大鼠均有明显降低,但仅MCAO组大鼠在1、5 d红细胞变形指数较正常组比较有差异(P<0.05)。但三组模型大鼠血液流变学统计学比较无明显差异(P>0.05)。结论在三种脑缺血模型中,大脑中动脉阻塞模型为最接近临床发病特征的、血液流变学变化明显的脑缺血模型。

Objective To explore the hemorheological changes of three kinds of rat models with cerebral ischemia and screen one model conforming to hemorheological changes and clinical features of cerebral ischemia. Methods Rats were divided into normal group and three cerebral ischemia groups, the middle cerebral artery occlusion with intraluminal thread group（MCAO-group）, the double common carotid artery ligation group（DCCA-group） and the single common carotid artery ligation （SCCA-group）. Blood for hemorheological day after , plasma the three cerebral ischemia groups and normal group（P 〈 0.05）. The whole blood viscosity, plasma viscosity and hematocrit of rats in MCAO-group were the highest on the 3rd day among the three model groups after cerebral ischemia, their values were（19.29 ± 2. 996） mPa s/5 s^-1, （1. 812 ± 0. 348） mPa s and （47.5 ± 5.28） %, respectively. While the deformity index of RBC of rats in MCAO-group was significantly lower than that in normal group on the 1 ^st day and 5^th day（P 〈 0.05）. There was no significant difference of other indexes of hemorheology of rats among the three cerebral ischemia groups（P 〉 0.05）. Conclusion Among the three models with cerebral ischemia, the middle cerebral artery occlusion model was the most suitable cerebral ischemia model,which shows obvious changes in hemorheology and it was consistent with the clinical features of stroke.