急性白血病端粒酶和乳酸脱氢酶活性检测的临床意义

Clinical sigificance of telomerase and serum lactic dehydrogenase activity in acute leukemia

目的探讨急性白血病端粒酶和乳酸脱氢酶活性的变化及其临床意义。方法选取38例急性白血病和14例非恶性血液系统疾病患者,采用端粒重复序列聚合酶链反应扩增免疫酶标法检测骨髓细胞端粒酶活性,同时用连续动态监测法测定血清乳酸脱氢酶浓度。结果急性白血病患者端粒酶活性和乳酸脱氢酶活性高于对照组非恶性血液系统疾病患者(P<0.01)。急性白血病初发及复发期患者高于完全缓解期患者(P<0.01及P<0.05)。结论观察急性白血病患者端粒酶和乳酸脱氢酶活性的变化有助于判定白血病疗效、预后及监测疾病的进展。

Objective To study the telomerase and serum lactic dehydregenase （LDH） activity in acute leukemia and explore its significance. Methods Thirty-eight cases of acute leukemia were studied, and fourteen cases of non-malignant hematologic disease were used as control. The telomerase activity was determined in bone marrow cell by TRAP-PCR-ELISA ,and LDH was detected in serum bofore chemotherapy or relapse and in complete remission period. Results Telomerase and serum LDH that activity were higher in acute leukemia than that in nonmalignant hematologic disease（ P 〈 0. 05 ） and higher in untreated patients with acute leukemia or patients who relapsed than that in complete remissive patients（ P 〈 0. 01 and P 〈 0. 05 ）. Conclusions The analysis of telomerase and serum LDH that activity might bo helpful to predict the therapeutic effect and the relapse of leukemia.