摘要
目的观察牛磺酸对庆大霉素引起的中毒性急性肾损伤的保护作用及其机制。方法将成年Wistar大鼠36只随机分为3组,牛磺酸组（n=12）：腹腔注射庆大霉素100 mg/kg,每天1次,共7 d,于每次注射前6 h腹腔注射100 g/L牛磺酸7.5 mL/kg;庆大霉素组（n=12）：同法注射庆大霉素,腹腔注射生理盐水代替牛磺酸;对照组（n=12）：用等量生理盐水代替庆大霉素及牛磺酸腹腔注射。检测各组大鼠24 h尿量（UV）、血肌酐（SCr）、血尿素氮（BUN）、肌酐清除率（CCr）及肾组织中内皮素-1（ET-1）的表达,观察肾组织形态学改变。结果牛磺酸组UV、BUN及SCr低于庆大霉素组（F=30.28～104.99,q=4.58～6.25,P〈0.01）,CCr高于庆大霉素组（F=22.46,q=5.06,P〈0.01）;庆大霉素组肾组织形态学损伤严重,牛磺酸组病变较轻（Hc=31.374,P〈0.001）;牛磺酸组肾小管上皮细胞中ET-1的表达明显弱于庆大霉素组（Hc=22.364～26.317,P〈0.001）。结论外源性牛磺酸可通过抑制ET-1的合成、释放对庆大霉素中毒性急性肾损伤发挥保护作用。
Objective To observe whether taurine provides the protective effect on gentamicin-induced toxic acute renal injury. Methods Adult Wistar rats of both sexes were divided into three groups randomly： Taurine group（n=12）,intraperitoneal injection of gentamicin（100 mg·kg^-1·d^-1）,once a day,for seven days,and 10% taurine solution（7.5 mL·kg^-1·d^-1） 6 hours before each gentamicin injection;Gentamicin group（n=12）,gentamicin given as the taurine group and the same amount of saline instead of taurine;Control group（n=12）,the same amount of saline instead of gentamicin and taurine.The urine volume（UV）,serum creatinine（SCr）,blood urea nitrogen（BUN）,creatinine clearance（CCr）;renal histomorphological changes and immunohistochemical expression of endothelin-1 in the kidney were observed. Results The UV,SCr and BUN levels of the taurine group were significantly lower than that of the gentamicin group（F=30.28-104.99,q=4.58-6.25,P〈0.01）,and the CCr level was increased by taurine（F=22.46,q=5.06,P〈0.001）.Histomorphologically,the gentamicin group revealed more severe damages than the taurine group did（Hc =31.374,P〈0.001）.Immunohistochemically,the expression of ET-1 in the gentamicin group was much more obvious than that in the taurine group（Hc=22.364-26.317,P〈0.001）. Conclusion Taurine is effective in protecting the morphology and function of kidneys of rats from gentamicin-induced acute renal injury by inhibiting synthesis and releasing ET-1.
出处
《齐鲁医学杂志》
2007年第5期377-379,共3页
Medical Journal of Qilu
